Histology-directed matrix-assisted laser desorption/ionization analysis reveals tissue origin and p53 status of primary liver cancers

Young Eun Jeon, Seok Cheol Lee, Seung Sam Paik, Kyeong Geun Lee, So Young Jin, Hyo Rim Kim, Chong Woo Yoo, Hye Min Park, Sang Yun Han, Dongho Choi, Hark Kyun Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To date, protein profiles for hepatocellular carcinomas and cholangiocarcinomas have not been systematically evaluated and compared with each other in an unbiased way. Thirty-six hepatocellular carcinomas and adjacent normal tissue samples were analyzed using histology-directed, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). Four cholangiocarcinomas and adjacent normal tissue samples were also evaluated. Tissue samples were sectioned at 10μm, with 1-3 sections thaw-mounted on a conductive indium tin oxide-coated glass slide. Sinapinic acid was manually deposited on areas of each tissue section enriched by epithelial cells, either tumor or normal, and mass spectra were acquired using a MALDI-time of flight instrument. According to class prediction analysis, average prediction accuracy in test sets (composed of 18 hepatocellular carcinoma-normal pairs) ranged from 93.0 to 95.8%. Cholangiocarcinomas and hepatocellular carcinomas had different protein profiles, as evidenced by average prediction accuracy of >95% in the test set for all classifiers. Permutation P-values for 0.632 + bootstrap cross validated misclassification rates (at feature selection P < 0.001) were less than 0.05 for predicting p53 immunostaining status. We conclude that MALDI MS profiles may be useful in assisting with the diagnosis and the differential diagnosis of primary liver cancers.

Original languageEnglish
Pages (from-to)449-455
Number of pages7
JournalPathology International
Volume61
Issue number8
DOIs
StatePublished - 2011 Aug 1

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Liver Neoplasms
Cholangiocarcinoma
Hepatocellular Carcinoma
Histology
Lasers
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Glass
Proteins
Differential Diagnosis
Epithelial Cells
Neoplasms

Keywords

  • Cholangiocarcinoma
  • Hepatocellular carcinoma
  • Histology directed MALDI

Cite this

Jeon, Young Eun ; Lee, Seok Cheol ; Paik, Seung Sam ; Lee, Kyeong Geun ; Jin, So Young ; Kim, Hyo Rim ; Yoo, Chong Woo ; Park, Hye Min ; Han, Sang Yun ; Choi, Dongho ; Kim, Hark Kyun. / Histology-directed matrix-assisted laser desorption/ionization analysis reveals tissue origin and p53 status of primary liver cancers. In: Pathology International. 2011 ; Vol. 61, No. 8. pp. 449-455.
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Histology-directed matrix-assisted laser desorption/ionization analysis reveals tissue origin and p53 status of primary liver cancers. / Jeon, Young Eun; Lee, Seok Cheol; Paik, Seung Sam; Lee, Kyeong Geun; Jin, So Young; Kim, Hyo Rim; Yoo, Chong Woo; Park, Hye Min; Han, Sang Yun; Choi, Dongho; Kim, Hark Kyun.

In: Pathology International, Vol. 61, No. 8, 01.08.2011, p. 449-455.

Research output: Contribution to journalArticle

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