Heterotrophic nitrification-aerobic denitrification potential of cyanide and thiocyanate degrading microbial communities under cyanogenic conditions

Lukhanyo Mekuto, Kimyoung Mo, Seteno K.O. Ntwampe, Maxwell Mewa-Ngongang, John Baptist N. Mudumbi, Nkosikho Dlangamandla, Elie Fereche Itoba-Tombo, E. A. Akinpelu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The impact of free cyanide (CN-) and thiocyanate (SCN-) on the CN- (CDO) and SCN- degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO’s were able to nitrify under cyanogenic conditions, achieving NH4 +-N removal rates above 1.66 mg NH4 +-N.L-1.h-1, except when CN- and SCN- loading was 15 mg CN-/L and 50 mg SCN-.L-1, respectively, which slightly inhibited nitrification. The TDO’s were able to achieve a nitrification rate of 1.59 mg NH4 +-N.L-1.h-1 in the absence of both CN- and SCN-, while the presence of CN- and SCN- was inhibitory, with a nitrification rates of 1.14 mg NH4 +-N.L-1.h-1. The CDO’s and TDO’s were able to denitrify aerobically, with the CDO’s obtaining NO3 --N removal rates above 0.67 mg NO3 --N.L-1.h-1, irrespective of the tested CN- and SCN- concentration range. Denitrification by the TDO’s was inhibited by CN-, achieving a removal rate of 0.46 mg NO3 --N.L-1.h-1 and 0.22 mg NO3 --N.L-1.h-1 when CN-concentration was 10 and 15 mg CN-.L-1, respectively. However, when the CDO’s and TDO’s were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg NH4 +-N.L-1.h-1 and 0.63 mg NO3 --N.L-1.h-1 irrespective of CN- and SCN- concentrations.

Original languageEnglish
Pages (from-to)254-262
Number of pages9
JournalEnvironmental Engineering Research
Volume24
Issue number2
DOIs
StatePublished - 2019 Jan 1

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Nitrification
Denitrification
Cyanides

Keywords

  • Aerobic denitrification
  • Cyanide degraders
  • Free cyanide
  • Nitrification
  • Thiocyanate
  • Thiocyanate degrader

Cite this

Mekuto, Lukhanyo ; Mo, Kimyoung ; Ntwampe, Seteno K.O. ; Mewa-Ngongang, Maxwell ; Mudumbi, John Baptist N. ; Dlangamandla, Nkosikho ; Itoba-Tombo, Elie Fereche ; Akinpelu, E. A. / Heterotrophic nitrification-aerobic denitrification potential of cyanide and thiocyanate degrading microbial communities under cyanogenic conditions. In: Environmental Engineering Research. 2019 ; Vol. 24, No. 2. pp. 254-262.
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abstract = "The impact of free cyanide (CN-) and thiocyanate (SCN-) on the CN- (CDO) and SCN- degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO’s were able to nitrify under cyanogenic conditions, achieving NH4 +-N removal rates above 1.66 mg NH4 +-N.L-1.h-1, except when CN- and SCN- loading was 15 mg CN-/L and 50 mg SCN-.L-1, respectively, which slightly inhibited nitrification. The TDO’s were able to achieve a nitrification rate of 1.59 mg NH4 +-N.L-1.h-1 in the absence of both CN- and SCN-, while the presence of CN- and SCN- was inhibitory, with a nitrification rates of 1.14 mg NH4 +-N.L-1.h-1. The CDO’s and TDO’s were able to denitrify aerobically, with the CDO’s obtaining NO3 --N removal rates above 0.67 mg NO3 --N.L-1.h-1, irrespective of the tested CN- and SCN- concentration range. Denitrification by the TDO’s was inhibited by CN-, achieving a removal rate of 0.46 mg NO3 --N.L-1.h-1 and 0.22 mg NO3 --N.L-1.h-1 when CN-concentration was 10 and 15 mg CN-.L-1, respectively. However, when the CDO’s and TDO’s were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg NH4 +-N.L-1.h-1 and 0.63 mg NO3 --N.L-1.h-1 irrespective of CN- and SCN- concentrations.",
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Heterotrophic nitrification-aerobic denitrification potential of cyanide and thiocyanate degrading microbial communities under cyanogenic conditions. / Mekuto, Lukhanyo; Mo, Kimyoung; Ntwampe, Seteno K.O.; Mewa-Ngongang, Maxwell; Mudumbi, John Baptist N.; Dlangamandla, Nkosikho; Itoba-Tombo, Elie Fereche; Akinpelu, E. A.

In: Environmental Engineering Research, Vol. 24, No. 2, 01.01.2019, p. 254-262.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Heterotrophic nitrification-aerobic denitrification potential of cyanide and thiocyanate degrading microbial communities under cyanogenic conditions

AU - Mekuto, Lukhanyo

AU - Mo, Kimyoung

AU - Ntwampe, Seteno K.O.

AU - Mewa-Ngongang, Maxwell

AU - Mudumbi, John Baptist N.

AU - Dlangamandla, Nkosikho

AU - Itoba-Tombo, Elie Fereche

AU - Akinpelu, E. A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The impact of free cyanide (CN-) and thiocyanate (SCN-) on the CN- (CDO) and SCN- degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO’s were able to nitrify under cyanogenic conditions, achieving NH4 +-N removal rates above 1.66 mg NH4 +-N.L-1.h-1, except when CN- and SCN- loading was 15 mg CN-/L and 50 mg SCN-.L-1, respectively, which slightly inhibited nitrification. The TDO’s were able to achieve a nitrification rate of 1.59 mg NH4 +-N.L-1.h-1 in the absence of both CN- and SCN-, while the presence of CN- and SCN- was inhibitory, with a nitrification rates of 1.14 mg NH4 +-N.L-1.h-1. The CDO’s and TDO’s were able to denitrify aerobically, with the CDO’s obtaining NO3 --N removal rates above 0.67 mg NO3 --N.L-1.h-1, irrespective of the tested CN- and SCN- concentration range. Denitrification by the TDO’s was inhibited by CN-, achieving a removal rate of 0.46 mg NO3 --N.L-1.h-1 and 0.22 mg NO3 --N.L-1.h-1 when CN-concentration was 10 and 15 mg CN-.L-1, respectively. However, when the CDO’s and TDO’s were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg NH4 +-N.L-1.h-1 and 0.63 mg NO3 --N.L-1.h-1 irrespective of CN- and SCN- concentrations.

AB - The impact of free cyanide (CN-) and thiocyanate (SCN-) on the CN- (CDO) and SCN- degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO’s were able to nitrify under cyanogenic conditions, achieving NH4 +-N removal rates above 1.66 mg NH4 +-N.L-1.h-1, except when CN- and SCN- loading was 15 mg CN-/L and 50 mg SCN-.L-1, respectively, which slightly inhibited nitrification. The TDO’s were able to achieve a nitrification rate of 1.59 mg NH4 +-N.L-1.h-1 in the absence of both CN- and SCN-, while the presence of CN- and SCN- was inhibitory, with a nitrification rates of 1.14 mg NH4 +-N.L-1.h-1. The CDO’s and TDO’s were able to denitrify aerobically, with the CDO’s obtaining NO3 --N removal rates above 0.67 mg NO3 --N.L-1.h-1, irrespective of the tested CN- and SCN- concentration range. Denitrification by the TDO’s was inhibited by CN-, achieving a removal rate of 0.46 mg NO3 --N.L-1.h-1 and 0.22 mg NO3 --N.L-1.h-1 when CN-concentration was 10 and 15 mg CN-.L-1, respectively. However, when the CDO’s and TDO’s were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg NH4 +-N.L-1.h-1 and 0.63 mg NO3 --N.L-1.h-1 irrespective of CN- and SCN- concentrations.

KW - Aerobic denitrification

KW - Cyanide degraders

KW - Free cyanide

KW - Nitrification

KW - Thiocyanate

KW - Thiocyanate degrader

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U2 - 10.4491/EER.2018.147

DO - 10.4491/EER.2018.147

M3 - Article

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JO - Environmental Engineering Research

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