Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro

Rizwan Wahab, Neha Kaushik, Farheen Khan, Nagendra Kumar Kaushik, Su Jae Lee, Eun Ha Choi, Abdulaziz A. Al-Khedhairy

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, <10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

Original languageEnglish
Pages (from-to)1131-1148
Number of pages18
JournalInternational Journal of Nanomedicine
Volume14
DOIs
StatePublished - 2019 Jan 1

Fingerprint

Catenins
Quantum Dots
Glioma
Gold
Semiconductor quantum dots
Tumors
Oncology
Stem Cells
Down-Regulation
Nanoparticles
Cells
Biostatistics
Neoplasms
Neoplastic Stem Cells
Cell growth
Stem cells
Diffraction patterns
Assays
Growth
Transmission electron microscopy

Keywords

  • Biostatistics
  • Brain cancer
  • Cellular movement
  • Epithelial-mesenchymal transition
  • Multivarient gold nanoparticles
  • Self-renewal
  • Solid tumor

Cite this

Wahab, Rizwan ; Kaushik, Neha ; Khan, Farheen ; Kaushik, Nagendra Kumar ; Lee, Su Jae ; Choi, Eun Ha ; Al-Khedhairy, Abdulaziz A. / Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro. In: International Journal of Nanomedicine. 2019 ; Vol. 14. pp. 1131-1148.
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abstract = "Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, <10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.",
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Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro. / Wahab, Rizwan; Kaushik, Neha; Khan, Farheen; Kaushik, Nagendra Kumar; Lee, Su Jae; Choi, Eun Ha; Al-Khedhairy, Abdulaziz A.

In: International Journal of Nanomedicine, Vol. 14, 01.01.2019, p. 1131-1148.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro

AU - Wahab, Rizwan

AU - Kaushik, Neha

AU - Khan, Farheen

AU - Kaushik, Nagendra Kumar

AU - Lee, Su Jae

AU - Choi, Eun Ha

AU - Al-Khedhairy, Abdulaziz A.

PY - 2019/1/1

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N2 - Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, <10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

AB - Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, <10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

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