Expression and characterization of codon-optimized carbonic anhydrase from dunaliella species for CO2 sequestration application

Bashistha Kumar Kanth, Kiha Min, Shipra Kumari, Hancheol Jeon, EonSeon Jin, Jinwon Lee, Seung Pil Pack

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Carbonic anhydrases (CAs) have been given much attention as biocatalysts for CO2 sequestration process because of their ability to convert CO2 to bicarbonate. Here, we expressed codon-optimized sequence of α-type CA cloned from Dunaliella species (Dsp-aCAopt) and characterized its catalyzing properties to apply for CO2 to calcite formation. The expressed amount of Dsp-aCAopt in Escherichia coli is about 50 mg/L via induction of 1.0 mM isopropyl-β-D-thiogalactopyranoside at 20 °C (for the case of intact Dsp-aCA, negligible). Dsp-aCAopt enzyme shows 47 °C of half-denaturation temperature and show wide pH stability (optimum pH 7.6/10.0). Apparent values of Km and Vmax for p-nitrophenylacetate substrate are 0.91 mM and 3.303×10-5 μM min-1. The effects of metal ions and anions were investigated to find out which factors enhance or inhibit Dsp-aCAopt activity. Finally, we demonstrated that Dsp-aCAopt enzyme can catalyze well the conversion of CO2 to CaCO3, as the calcite form, in the Ca2+ solution [8.9 mg/100 μg (172 U/mg enzyme) with 10 mM of Ca2+]. The obtained expression and characterization results of Dsp-aCAopt would be usefully employed for the development of efficient CA-based system for CO2-converting/capturing processes.

Original languageEnglish
Pages (from-to)2341-2356
Number of pages16
JournalApplied Biochemistry and Biotechnology
Issue number8
StatePublished - 2012 Aug 1


  • Alpha-carbonic anhydrase
  • CO2 sequestration
  • Codon optimization
  • Dunaliella species
  • Inhibitory effects

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