Enhancement of cardiac myoblast responses onto electrospun PLCL fibrous matrices coated with polydopamine for gelatin immobilization

Young Min Shin, Hansoo Park, Heungsoo Shin

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A variety of surface modification techniques have been proposed to improve the cell-biomaterial interactions. On the other hand, these processes may cleave long-chained polymers, and compromise their mechanical properties. In this study, dopamine was used as a bridge molecule to immobilize gelatin on the poly(L-lactide-co-ω- caprolactone) (PLCL) fibrous matrices, which may then be used as a cell delivery carrier. The PLCL fibrous matrices coated with polydopamine by dipping (D-PLCL) can subsequently immobilize gelatin (GD-PLCL). The D-PLCL matrices showed minimal changes in the mechanical properties with a tensile strain of 251.0±33.4% and 247.8±32.1% before and after the coating process, respectively. The cellular activities on the fibrous matrices increased in the order of PLCL<G-PLCL<D-PLCL<GD-PLCL; the H9c2 myoblasts on the GD-PLCL matrices showed approximately two-times higher adhesion and spreading than those on the PLCL matrices, and the proliferation was significantly greater on the GD-PLCL matrices than on the other matrices. Therefore, polydopamine can effectively immobilize the bioactive functional groups on the surface of electrospun fibrous matrices for the development of a tissue specific cell delivery carrier.

Original languageEnglish
Pages (from-to)835-842
Number of pages8
JournalMacromolecular Research
Volume19
Issue number8
DOIs
StatePublished - 2011 Aug 1

Keywords

  • Electrospinning
  • Gelatin
  • Polydopamine
  • Surface modification

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