Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture

Changsun Kim, Hansol Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. Methods: In this study, which was conducted at the emergency department of a tertiary care hospital in April–July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc® system, USA), an arterial blood gas analyzer (pHOx®Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. Results: The pH, HCO 3 , Ca 2+ , Na + , K + , Cl , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95% confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥ 80%). Of the remaining three parameters (pCO 2 pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 = 0.824, y = − 1.411 + 0.877·x; lactate: R 2 = 0.902, y = − 0.544 + 0.966·x; pO 2 : R 2 = 0.037, y = 61.6 + 0.431·x). Conclusion: Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method.

Original languageEnglish
Pages (from-to)1215-1221
Number of pages7
JournalAmerican Journal of Emergency Medicine
Volume36
Issue number7
DOIs
StatePublished - 2018 Jul 1

Fingerprint

Point-of-Care Systems
Emergency Medical Technicians
Punctures
Skin
Lactic Acid
Reference Values
Tertiary Healthcare
Tertiary Care Centers
Blood Vessels
Hospital Emergency Service
Linear Models
Gases
Confidence Intervals
Glucose
Equipment and Supplies

Keywords

  • Capillary blood
  • Emergency medical services
  • Point-of-care systems

Cite this

@article{a9ca073b39d343d3a20fb828be335d2c,
title = "Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture",
abstract = "Objective: Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. Methods: In this study, which was conducted at the emergency department of a tertiary care hospital in April–July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc{\circledR} system, USA), an arterial blood gas analyzer (pHOx{\circledR}Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. Results: The pH, HCO 3 , Ca 2+ , Na + , K + , Cl − , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95{\%} confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥ 80{\%}). Of the remaining three parameters (pCO 2 pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 = 0.824, y = − 1.411 + 0.877·x; lactate: R 2 = 0.902, y = − 0.544 + 0.966·x; pO 2 : R 2 = 0.037, y = 61.6 + 0.431·x). Conclusion: Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method.",
keywords = "Capillary blood, Emergency medical services, Point-of-care systems",
author = "Changsun Kim and Hansol Kim",
year = "2018",
month = "7",
day = "1",
doi = "10.1016/j.ajem.2017.12.025",
language = "English",
volume = "36",
pages = "1215--1221",
journal = "American Journal of Emergency Medicine",
issn = "0735-6757",
number = "7",

}

Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture. / Kim, Changsun; Kim, Hansol.

In: American Journal of Emergency Medicine, Vol. 36, No. 7, 01.07.2018, p. 1215-1221.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Emergency medical technician-performed point-of-care blood analysis using the capillary blood obtained from skin puncture

AU - Kim, Changsun

AU - Kim, Hansol

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Objective: Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. Methods: In this study, which was conducted at the emergency department of a tertiary care hospital in April–July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc® system, USA), an arterial blood gas analyzer (pHOx®Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. Results: The pH, HCO 3 , Ca 2+ , Na + , K + , Cl − , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95% confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥ 80%). Of the remaining three parameters (pCO 2 pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 = 0.824, y = − 1.411 + 0.877·x; lactate: R 2 = 0.902, y = − 0.544 + 0.966·x; pO 2 : R 2 = 0.037, y = 61.6 + 0.431·x). Conclusion: Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method.

AB - Objective: Comparing a point-of-care (POC) test using the capillary blood obtained from skin puncture with conventional laboratory tests. Methods: In this study, which was conducted at the emergency department of a tertiary care hospital in April–July 2017, 232 patients were enrolled, and three types of blood samples (capillary blood from skin puncture, arterial and venous blood from blood vessel puncture) were simultaneously collected. Each blood sample was analyzed using a POC analyzer (epoc® system, USA), an arterial blood gas analyzer (pHOx®Ultra, Nova biomedical, USA) and venous blood analyzers (AU5800, DxH2401, Beckman Coulter, USA). Twelve parameters were compared between the epoc and reference analyzers, with an equivalence test, Bland-Altman plot analysis and linear regression employed to show the agreement or correlation between the two methods. Results: The pH, HCO 3 , Ca 2+ , Na + , K + , Cl − , glucose, Hb and Hct measured by the epoc were equivalent to the reference values (95% confidence interval of mean difference within the range of the agreement target) with clinically inconsequential mean differences and narrow limits of agreement. All of them, except pH, had clinically acceptable agreements between the two methods (results within target value ≥ 80%). Of the remaining three parameters (pCO 2 pO 2 and lactate), the epoc pCO 2 and lactate values were highly correlated with the reference device values, whereas pO 2 was not. (pCO 2 : R 2 = 0.824, y = − 1.411 + 0.877·x; lactate: R 2 = 0.902, y = − 0.544 + 0.966·x; pO 2 : R 2 = 0.037, y = 61.6 + 0.431·x). Conclusion: Most parameters, except only pO 2 , measured by the epoc were equivalent to or correlated with those from the reference method.

KW - Capillary blood

KW - Emergency medical services

KW - Point-of-care systems

UR - http://www.scopus.com/inward/record.url?scp=85042853439&partnerID=8YFLogxK

U2 - 10.1016/j.ajem.2017.12.025

DO - 10.1016/j.ajem.2017.12.025

M3 - Article

C2 - 29519760

AN - SCOPUS:85042853439

VL - 36

SP - 1215

EP - 1221

JO - American Journal of Emergency Medicine

JF - American Journal of Emergency Medicine

SN - 0735-6757

IS - 7

ER -