Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells

Yong Hee Rhee, Lesly Puspita, Yanuar Alan Sulistio, Seung Won Kim, Vincencius Vidyawan, Rosalie Elvira, Mi Yoon Chang, Jae won Shim, Sang-Hun Lee

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Rhee et al. report that residual expression of OCT3/4 in NPC cultures disappears within days when cultured under neural differentiation culture condition. The authors further propose that hPSCs co-cultured with hESC-derived NPCs become NPCs or neurons in an efficient and reproducible manner across multiple hPSC lines.

Original languageEnglish
JournalMolecular Therapy
DOIs
StatePublished - 2019 Jan 1

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Neurons
Human Embryonic Stem Cells

Keywords

  • co-culture
  • human pluripotent stem cells
  • neural differentiation
  • neural precursor cells

Cite this

Rhee, Yong Hee ; Puspita, Lesly ; Sulistio, Yanuar Alan ; Kim, Seung Won ; Vidyawan, Vincencius ; Elvira, Rosalie ; Chang, Mi Yoon ; Shim, Jae won ; Lee, Sang-Hun. / Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells. In: Molecular Therapy. 2019.
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Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells. / Rhee, Yong Hee; Puspita, Lesly; Sulistio, Yanuar Alan; Kim, Seung Won; Vidyawan, Vincencius; Elvira, Rosalie; Chang, Mi Yoon; Shim, Jae won; Lee, Sang-Hun.

In: Molecular Therapy, 01.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Rhee, Yong Hee

AU - Puspita, Lesly

AU - Sulistio, Yanuar Alan

AU - Kim, Seung Won

AU - Vidyawan, Vincencius

AU - Elvira, Rosalie

AU - Chang, Mi Yoon

AU - Shim, Jae won

AU - Lee, Sang-Hun

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