Interleukin-2(IL-2) is a T cell derived lymphokine with a broad spectrum of growth and differentiation promoting activities. Lymphokine-activated killer(LAK) cells are generated by culture of fresh peripheral blood lymphocytes (PBL) with IL-2. LAK cells can lyse a wide spectrum of allogeneic and syngeneic tumor cells. Recently, much attention has been paid to clinical trials of adoptive immunotherapy with LAK cells for treatment of cancer. Little is known, however, about the mechanisms regulating LAK cell induction by IL-2. Interferon(IFN) was discovered by virtue of its direct antiviral activity, but today IFNs are known to influence a wide variety of activities of the immune system. To examine the effects of two classes of IFNs(IFN-α 2A and IFN-γ) on LAK activity induced by IL-2, we isolated human PBL from healthy donors and added various kinds of IFNs to cultures of lymphocytes with IL-2. Addition of exogenous IFN-α 2A to cultures of lymphocytes plus IL-2 inhibited LAK activity, but addition of IFN-γ had no effect on LAK induction by IL-2. IFN-α 2A also inhibited the proliferative responses of lymphocytes to IL-2 stimulation and the expression of IL-2 receptors on their surface, whereas IFN-γ did not. Our results can provide useful information to adoptive anticancer immunotherapy.
|Number of pages||10|
|Journal||Journal of the Korean Society for Microbiology|
|State||Published - 1992|