Effect of sildenafil citrate on interleukin-1β-induced nitric oxide synthesis and iNOS expression in SW982 cells

Kyung Ok Kim, Shin Young Park, Chang Woo Han, Kee Chung Hyun, Dae Hyun Ryu, Joong Soo Han

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of this study was to identify the effect of sildenafil citrate on IL-1β-induced nitric oxide (NO) synthesis and iNOS expression in human synovial sarcoma SW982 cells. IL-1β stimulated the cells to generate NO in both dose- and time-dependent manners. The IL-1β-induced NO synthesis was inhibited by guanylate cyclase (GC) inhibitor, LY83583. When the cells were treated with 8-bromo-cGMP, a hydrolyzable analog of cGMP, NO synthesis was increased upto 5-fold without IL-1β treatment suggesting that cGMP is an essential component for increasing the NO synthesis. Synoviocytes and chondrocytes contain strong cGMP phosphodiesterase (PDE) activity, which has biochemical features of PDE5. When SW982 cells were pretreated with sildenafil citrate (Viagra), a PDE5 specific inhibitor, sildenafil citrate significantly inhibited IL-1β-induced NO synthesis and iNOS expressions. From this result, we noticed that PDE5 activity is required for IL-1β-induced NO synthesis and iNOS expressions in human synovial sarcoma cells, and sildenafil citrate may be able to suppress an inflammatory reaction of synovium through inhibition of NO synthesis and iNOS expression by cytokines.

Original languageEnglish
Pages (from-to)286-293
Number of pages8
JournalExperimental and Molecular Medicine
Volume40
Issue number3
DOIs
StatePublished - 2008 Jun 30

Fingerprint

Interleukin-1
Nitric Oxide
Synovial Sarcoma
6-anilino-5,8-quinolinedione
Phosphodiesterase 5 Inhibitors
Sildenafil Citrate
Synovial Membrane
Guanylate Cyclase
Phosphoric Diester Hydrolases
Chondrocytes
Cytokines

Keywords

  • Cyclic nucleotide phosphodiesterases, type 5
  • Interleukin-1β
  • Nitric oxide
  • Nitric oxide synthase type II
  • Sildenafil

Cite this

Kim, Kyung Ok ; Park, Shin Young ; Han, Chang Woo ; Hyun, Kee Chung ; Ryu, Dae Hyun ; Han, Joong Soo. / Effect of sildenafil citrate on interleukin-1β-induced nitric oxide synthesis and iNOS expression in SW982 cells. In: Experimental and Molecular Medicine. 2008 ; Vol. 40, No. 3. pp. 286-293.
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Effect of sildenafil citrate on interleukin-1β-induced nitric oxide synthesis and iNOS expression in SW982 cells. / Kim, Kyung Ok; Park, Shin Young; Han, Chang Woo; Hyun, Kee Chung; Ryu, Dae Hyun; Han, Joong Soo.

In: Experimental and Molecular Medicine, Vol. 40, No. 3, 30.06.2008, p. 286-293.

Research output: Contribution to journalArticle

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