Differentiation stage alters matrix control of stem cells

Susan X. Hsiong, Paolo Carampin, Hyun Joon Kong, Kuen Yong Lee, David J. Mooney

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Cues from the material to which a cell is adherent (e.g., adhesion ligand presentation, substrate elastic modulus) clearly influence the phenotype of differentiated cells. However, it is currently unclear if stem cells respond similarly to these cues. This study examined how the overall density and nanoscale organization of a model cell adhesion ligand (arginine-glycine- aspartic acid [RGD] containing peptide) presented from hydrogels of varying stiffness regulated the proliferation of a clonally derived stem cell line (D1 cells) and preosteoblasts (MC3T3-E1). While the growth rate of MC3T3-E1 preosteoblasts was responsive to nanoscale RGD ligand organization and substrate stiffness, the D1 stem cells were less sensitive to these cues in their uncommitted state. However, once the D1 cells were differentiated towards the osteoblast lineage, they became more responsive to these signals. These results demonstrate that the cell response to material cues is dependent on the stage of cell commitment or differentiation, and these findings will likely impact the design of biomaterials for tissue regeneration.

Original languageEnglish
Pages (from-to)145-156
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume85
Issue number1
DOIs
StatePublished - 2008 Apr 1

Fingerprint

Stem cells
Ligands
Stiffness
Tissue regeneration
Arginine
Hydrogels
Osteoblasts
Cell adhesion
Biocompatible Materials
Substrates
Aspartic Acid
Biomaterials
Glycine
Peptides
Amino acids
Adhesion
Elastic moduli
Acids

Keywords

  • Biomaterial
  • Cell differentiation
  • Extracellular matrix
  • Osteoblasts
  • Stem cells

Cite this

Hsiong, Susan X. ; Carampin, Paolo ; Kong, Hyun Joon ; Lee, Kuen Yong ; Mooney, David J. / Differentiation stage alters matrix control of stem cells. In: Journal of Biomedical Materials Research - Part A. 2008 ; Vol. 85, No. 1. pp. 145-156.
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Differentiation stage alters matrix control of stem cells. / Hsiong, Susan X.; Carampin, Paolo; Kong, Hyun Joon; Lee, Kuen Yong; Mooney, David J.

In: Journal of Biomedical Materials Research - Part A, Vol. 85, No. 1, 01.04.2008, p. 145-156.

Research output: Contribution to journalArticle

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