Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS

Jeonghoon Han, Eun Ji Won, Min Chul Lee, Jung Soo Seo, Su Jae Lee, Jae Seong Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctane sulfonate (PFOS) are widely dispersed persistent organic pollutants (POPs) in the marine ecosystem. However, their toxic effects on marine organisms are still poorly understood. In this study, we investigated the effects of BDE-47 and PFOS on development and reproduction at the organismal level and reactive oxygen species (ROS) production and gene expression patterns of the defensome at the cellular level in the intertidal copepod Tigriopus japonicus. In copepods exposed to BDE-47 and PFOS, we observed developmental retardation and reduced fecundity, suggesting repercussions on in vivo endpoints through alterations to the normal molting and reproduction system of T. japonicus. BDE-47 and PFOS increased levels of ROS in T. japonicus in a concentration-dependent manner, indicating that POPs can induce oxidative stress through the generation of ROS. Additionally, transcript profiles of genes related to detoxification (. e.g., CYPs), antioxidant functions (. e.g., GST- sigma, catalase, MnSOD), apoptosis (. e.g., p53, Rb), and cellular proliferation (. e.g., PCNA) were modulated over 72. h in response to BDE-47 (120. μg/L) and PFOS (1000. μg/L). These findings indicate that BDE-47 and PFOS can induce oxidative stress-mediated DNA damage repair systems with transcriptional regulation of detoxification, antioxidant, and apoptosis-related genes, resulting in developmental retardation and reduced fecundity in the copepod T. japonicus.

Original languageEnglish
Pages (from-to)136-143
Number of pages8
JournalAquatic Toxicology
Volume165
DOIs
StatePublished - 2015 Aug 1

Fingerprint

Copepoda
sulfonate
Fertility
fecundity
persistent organic pollutants
reactive oxygen species
apoptosis
Reactive Oxygen Species
detoxification
antioxidant
oxidative stress
Reproduction
Oxidative Stress
Antioxidants
antioxidants
Apoptosis
gene
Molting
Aquatic Organisms
endpoints

Keywords

  • Copepod
  • Developmental retardation
  • Persistent organic pollutants
  • Reactive oxygen species
  • Reduced fecundity
  • Tigriopus japonicus

Cite this

@article{454faa5e20aa488bb832341c8b29ac26,
title = "Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS",
abstract = "2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctane sulfonate (PFOS) are widely dispersed persistent organic pollutants (POPs) in the marine ecosystem. However, their toxic effects on marine organisms are still poorly understood. In this study, we investigated the effects of BDE-47 and PFOS on development and reproduction at the organismal level and reactive oxygen species (ROS) production and gene expression patterns of the defensome at the cellular level in the intertidal copepod Tigriopus japonicus. In copepods exposed to BDE-47 and PFOS, we observed developmental retardation and reduced fecundity, suggesting repercussions on in vivo endpoints through alterations to the normal molting and reproduction system of T. japonicus. BDE-47 and PFOS increased levels of ROS in T. japonicus in a concentration-dependent manner, indicating that POPs can induce oxidative stress through the generation of ROS. Additionally, transcript profiles of genes related to detoxification (. e.g., CYPs), antioxidant functions (. e.g., GST- sigma, catalase, MnSOD), apoptosis (. e.g., p53, Rb), and cellular proliferation (. e.g., PCNA) were modulated over 72. h in response to BDE-47 (120. μg/L) and PFOS (1000. μg/L). These findings indicate that BDE-47 and PFOS can induce oxidative stress-mediated DNA damage repair systems with transcriptional regulation of detoxification, antioxidant, and apoptosis-related genes, resulting in developmental retardation and reduced fecundity in the copepod T. japonicus.",
keywords = "Copepod, Developmental retardation, Persistent organic pollutants, Reactive oxygen species, Reduced fecundity, Tigriopus japonicus",
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day = "1",
doi = "10.1016/j.aquatox.2015.05.022",
language = "English",
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journal = "Aquatic Toxicology",
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Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS. / Han, Jeonghoon; Won, Eun Ji; Lee, Min Chul; Seo, Jung Soo; Lee, Su Jae; Lee, Jae Seong.

In: Aquatic Toxicology, Vol. 165, 01.08.2015, p. 136-143.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Developmental retardation, reduced fecundity, and modulated expression of the defensome in the intertidal copepod Tigriopus japonicus exposed to BDE-47 and PFOS

AU - Han, Jeonghoon

AU - Won, Eun Ji

AU - Lee, Min Chul

AU - Seo, Jung Soo

AU - Lee, Su Jae

AU - Lee, Jae Seong

PY - 2015/8/1

Y1 - 2015/8/1

N2 - 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctane sulfonate (PFOS) are widely dispersed persistent organic pollutants (POPs) in the marine ecosystem. However, their toxic effects on marine organisms are still poorly understood. In this study, we investigated the effects of BDE-47 and PFOS on development and reproduction at the organismal level and reactive oxygen species (ROS) production and gene expression patterns of the defensome at the cellular level in the intertidal copepod Tigriopus japonicus. In copepods exposed to BDE-47 and PFOS, we observed developmental retardation and reduced fecundity, suggesting repercussions on in vivo endpoints through alterations to the normal molting and reproduction system of T. japonicus. BDE-47 and PFOS increased levels of ROS in T. japonicus in a concentration-dependent manner, indicating that POPs can induce oxidative stress through the generation of ROS. Additionally, transcript profiles of genes related to detoxification (. e.g., CYPs), antioxidant functions (. e.g., GST- sigma, catalase, MnSOD), apoptosis (. e.g., p53, Rb), and cellular proliferation (. e.g., PCNA) were modulated over 72. h in response to BDE-47 (120. μg/L) and PFOS (1000. μg/L). These findings indicate that BDE-47 and PFOS can induce oxidative stress-mediated DNA damage repair systems with transcriptional regulation of detoxification, antioxidant, and apoptosis-related genes, resulting in developmental retardation and reduced fecundity in the copepod T. japonicus.

AB - 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctane sulfonate (PFOS) are widely dispersed persistent organic pollutants (POPs) in the marine ecosystem. However, their toxic effects on marine organisms are still poorly understood. In this study, we investigated the effects of BDE-47 and PFOS on development and reproduction at the organismal level and reactive oxygen species (ROS) production and gene expression patterns of the defensome at the cellular level in the intertidal copepod Tigriopus japonicus. In copepods exposed to BDE-47 and PFOS, we observed developmental retardation and reduced fecundity, suggesting repercussions on in vivo endpoints through alterations to the normal molting and reproduction system of T. japonicus. BDE-47 and PFOS increased levels of ROS in T. japonicus in a concentration-dependent manner, indicating that POPs can induce oxidative stress through the generation of ROS. Additionally, transcript profiles of genes related to detoxification (. e.g., CYPs), antioxidant functions (. e.g., GST- sigma, catalase, MnSOD), apoptosis (. e.g., p53, Rb), and cellular proliferation (. e.g., PCNA) were modulated over 72. h in response to BDE-47 (120. μg/L) and PFOS (1000. μg/L). These findings indicate that BDE-47 and PFOS can induce oxidative stress-mediated DNA damage repair systems with transcriptional regulation of detoxification, antioxidant, and apoptosis-related genes, resulting in developmental retardation and reduced fecundity in the copepod T. japonicus.

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