Development of efficient adeno-associated virus (AAV)-mediated gene delivery system with a phytoactive material for targeting human melanoma cells

John Hwan Lee, Yoojin Kim, Ye Eun Yoon, Yong Jin Kim, Seong-Geun Oh, Jae Hyung Jang, Eunmi Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We exploited the emerging potential of gene therapy strategies to design a powerful therapeutic system that combines two key components—AAV vector and [6]-gingerol. In this study, we created an AAV2 construct expressing the proapoptotic protein BIM, which uses HSPG as its primary receptor, to target HSPG-overexpressing melanoma cells. This combination treatment showed promising results in vitro, inducing apoptosis in human melanoma cells. This new platform technology will make a significant contribution to numerous therapeutic applications, most notably for melanoma, including overcoming resistance to conventional anticancer therapies.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalNew Biotechnology
Volume37
DOIs
StatePublished - 2017 Jul 25

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Gene therapy
Gene Transfer Techniques
Heparan Sulfate Proteoglycans
Dependovirus
Cell death
Viruses
Melanoma
Genes
Proteins
Apoptosis
Therapeutics
Genetic Therapy
Technology
gingerol

Keywords

  • Adeno-associated virus
  • Gene delivery system
  • Melanoma
  • Pro-apoptotic gene
  • [6]-Gingerol

Cite this

Lee, John Hwan ; Kim, Yoojin ; Yoon, Ye Eun ; Kim, Yong Jin ; Oh, Seong-Geun ; Jang, Jae Hyung ; Kim, Eunmi. / Development of efficient adeno-associated virus (AAV)-mediated gene delivery system with a phytoactive material for targeting human melanoma cells. In: New Biotechnology. 2017 ; Vol. 37. pp. 194-199.
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Development of efficient adeno-associated virus (AAV)-mediated gene delivery system with a phytoactive material for targeting human melanoma cells. / Lee, John Hwan; Kim, Yoojin; Yoon, Ye Eun; Kim, Yong Jin; Oh, Seong-Geun; Jang, Jae Hyung; Kim, Eunmi.

In: New Biotechnology, Vol. 37, 25.07.2017, p. 194-199.

Research output: Contribution to journalArticle

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