Development and characterization of nanofibrous poly(lactic-co-glycolic acid)/biphasic calcium phosphate composite scaffolds for enhanced osteogenic differentiation

Ji Hye Lee, Yu Bin Lee, Nae Gyune Rim, Sun Young Jo, Youn Mook Lim, Heungsoo Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Poly(lactic-co-glycolic acid)(PLGA)/biphasic calcium phosphate (BCP) composite nanofibers with different BCP to PLGA ratios were fabricated using the electrospinning technique. The scanning electron microscopy (SEM) images showed a similar morphology and fibers in all groups. The incorporated BCP was dispersed homogenously throughout the nanofibers, and the surface roughness was affected by the input amount of BCP. The increase in amount of BCP incorporated was confirmed by several methods. BCP incorporation into the PLGA nanofibers did not affect the initial adhesion of osteoblasts and their adherent morphology. However, the proliferation of the cells cultured on the composite nanofibers for 10 days with larger amounts of BCP was delayed, suggesting that incorporated BCP may facilitate the switch from proliferation to differentiation of the osteoblasts. The incorporation of BCP enhanced the expression of osteogenic genes, as well as induced calcium deposition by the osteoblasts in the extracellular matrix(ECM) after 21 days of culture on the PLGA/BCP composite nanofibers. Overall, these results can provide evidence of the potential of BCP incorporation into the biomaterials for effective bone regeneration.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalMacromolecular Research
Volume19
Issue number2
DOIs
StatePublished - 2011 Feb 1

Fingerprint

Calcium phosphate
Scaffolds
Acids
Composite materials
Nanofibers
Osteoblasts
Milk
hydroxyapatite-beta tricalcium phosphate
polylactic acid-polyglycolic acid copolymer
Electrospinning
Biocompatible Materials
Biomaterials
Calcium
Bone
Adhesion
Genes
Surface roughness
Switches
Scanning electron microscopy

Keywords

  • biphasic calcium phosphate
  • composite nanofibers
  • osteoblast
  • poly(lactic-co-glycolic acid)

Cite this

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abstract = "Poly(lactic-co-glycolic acid)(PLGA)/biphasic calcium phosphate (BCP) composite nanofibers with different BCP to PLGA ratios were fabricated using the electrospinning technique. The scanning electron microscopy (SEM) images showed a similar morphology and fibers in all groups. The incorporated BCP was dispersed homogenously throughout the nanofibers, and the surface roughness was affected by the input amount of BCP. The increase in amount of BCP incorporated was confirmed by several methods. BCP incorporation into the PLGA nanofibers did not affect the initial adhesion of osteoblasts and their adherent morphology. However, the proliferation of the cells cultured on the composite nanofibers for 10 days with larger amounts of BCP was delayed, suggesting that incorporated BCP may facilitate the switch from proliferation to differentiation of the osteoblasts. The incorporation of BCP enhanced the expression of osteogenic genes, as well as induced calcium deposition by the osteoblasts in the extracellular matrix(ECM) after 21 days of culture on the PLGA/BCP composite nanofibers. Overall, these results can provide evidence of the potential of BCP incorporation into the biomaterials for effective bone regeneration.",
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Development and characterization of nanofibrous poly(lactic-co-glycolic acid)/biphasic calcium phosphate composite scaffolds for enhanced osteogenic differentiation. / Lee, Ji Hye; Lee, Yu Bin; Rim, Nae Gyune; Jo, Sun Young; Lim, Youn Mook; Shin, Heungsoo.

In: Macromolecular Research, Vol. 19, No. 2, 01.02.2011, p. 172-179.

Research output: Contribution to journalArticle

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