Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model

Ji Won Park, Sook Hee Ku, Hyung Ho Moon, Minhyung Lee, Donghoon Choi, Jaemoon Yang, Yong Min Huh, Ji Hoon Jeong, Tae Gwan Park, Hyejung Mok, Sun Hwa Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered MSCs are also clearly visualized via MR imaging after administration to rat cerebrovascular ischemia models, which enable tracking of MSCs migration from injected sites to injured ischemic area. Spherically cross-linked iron oxide nanoparticles (IONs) via catechol-grafted poly-L-lysine (PLL-DN) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) by magnet-mediated transfection of vascular endothelial growth factor plasmid DNA. They also enable monitoring of the MSC migration from injected site to ischemic region in rat cerebrovascular ischemia models via magnetic resonance (MR) imaging.

Original languageEnglish
Pages (from-to)380-389
Number of pages10
JournalMacromolecular Bioscience
Volume14
Issue number3
DOIs
StatePublished - 2014 Jan 1

Fingerprint

Stem cells
Brain Ischemia
Mesenchymal Stromal Cells
Iron oxides
Nanoparticles
Monitoring
Plasmids
DNA
Magnetic resonance
Vascular Endothelial Growth Factor A
Magnetic Resonance Imaging
Imaging techniques
Therapeutics
Cell Movement
Rats
Ischemia
Ethylene Glycol
Magnets
Magnetic Fields
Phase locked loops

Keywords

  • cell engineering
  • cerebral ischemia model
  • cross-linked iron oxide nanoparticles
  • magnetic resonance imaging
  • mesenchymal stem cells

Cite this

Park, Ji Won ; Ku, Sook Hee ; Moon, Hyung Ho ; Lee, Minhyung ; Choi, Donghoon ; Yang, Jaemoon ; Huh, Yong Min ; Jeong, Ji Hoon ; Park, Tae Gwan ; Mok, Hyejung ; Kim, Sun Hwa. / Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model. In: Macromolecular Bioscience. 2014 ; Vol. 14, No. 3. pp. 380-389.
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Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model. / Park, Ji Won; Ku, Sook Hee; Moon, Hyung Ho; Lee, Minhyung; Choi, Donghoon; Yang, Jaemoon; Huh, Yong Min; Jeong, Ji Hoon; Park, Tae Gwan; Mok, Hyejung; Kim, Sun Hwa.

In: Macromolecular Bioscience, Vol. 14, No. 3, 01.01.2014, p. 380-389.

Research output: Contribution to journalArticle

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AU - Yang, Jaemoon

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