CRISPR-Cas9 based genome editing for defective gene correction in humans and other mammals

Janardhan Keshav Karapurkar, Ainsley Mike Antao, Kye Seong Kim, Suresh Ramakrishna

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Clustered regularly interspaced short palindromic repeat-Cas9 (CRISPR/Cas9), derived from bacterial and archean immune systems, has received much attention from the scientific community as a powerful, targeted gene editing tool. The CRISPR/Cas9 system enables a simple, relatively effortless and highly specific gene targeting strategy through temporary or permanent genome regulation or editing. This endonuclease has enabled gene correction by taking advantage of the endogenous homology directed repair (HDR) pathway to successfully target and correct disease-causing gene mutations. Numerous studies using CRISPR support the promise of efficient and simple genome manipulation, and the technique has been validated in in vivo and in vitro experiments, indicating its potential for efficient gene correction at any genomic loci. In this chapter, we detailed various strategies related to gene editing using the CRISPR/Cas9 system. We also outlined strategies to improve the efficiency of gene correction via the HDR pathway and to improve viral and non-viral mediated gene delivery methods, with an emphasis on their therapeutic potential for correcting genetic disorder in humans and other mammals.

Original languageEnglish
Title of host publicationReprogramming the Genome
Subtitle of host publicationCRISPR-Cas-based Human Disease Therapy
EditorsVijai Singh
PublisherElsevier B.V.
Number of pages45
ISBN (Print)9780323853231
StatePublished - 2021 Jan

Publication series

NameProgress in Molecular Biology and Translational Science
ISSN (Print)1877-1173
ISSN (Electronic)1878-0814


  • Chromosomal inversion
  • Disease modeling
  • Gene defect
  • HDR
  • Monogenic disorder
  • NHEJ
  • iPSCs
  • ssODN


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