CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral integration sites

Heon Seok Kim, Gue Ho Hwang, Hyomin K. Lee, Taegeun Bae, Seong Ho Park, Yong Jun Kim, Sun Lee, Jae Hoon Park, Sangsu Bae, Junho K. Hur

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The study demonstrates a new technology “CReVIS-seq” that enables CRISPR-based multiplexed enrichment of genomic regions. The method uses a PCR-free enrichment step based on DNA circularization and precise CRISPR-Cas9 cleavage. The strength of this method was demonstrated by simultaneous enrichment of lentiviral integration sites and genomic sites.

Original languageEnglish
Pages (from-to)792-800
Number of pages9
JournalMolecular Therapy - Methods and Clinical Development
Volume20
DOIs
StatePublished - 2021 Mar 12

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