Conserved nature of Helicoverpa armigera gut bacterial flora on different host plants and in vitro interactions with PI proteins advocates role in host digestive physiology

Ashok A. Shinde, Faiyaz K. Shaikh, Prafull P. Gadge, Manohar V. Padul, Sanjay Prabhu Govindwar, Manvendra S. Kachole

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Helicoverpa armigera is anxious insect pest of agricultural crops. Array of defensive molecules in host plants and extensive use of chemical insecticides are unable to cease the attack incidences. Gut bacterial communities are found to contribute in various physiological activities in most of the arthropods. In the current study the bacterial communities were isolated from gut of H. armigera feeding on three host plants (Pigeonpea, Chickpea and Cotton) by culture dependent and culture independent methods. Predominant bacterial communities were identified by terminal restriction fragment length polymorphism (TRFLP). Three dominant phylotypes namely proteobacteria, actinobacteria and firmicutes were identified by TRFLP and found to conserve on different host plant selected. Five Bacillus species namely Bacillus sp. JR14, Bacillus sp. YP1, Bacillus safensis CG1, Bacillus subtillis KAVK2 and Bacillus megaterium 47N were purified by culture dependent method and identified by 16S rRNA sequencing. Among all identified Bacillus, Bacillus sp. YP1 strain was found to be potent protease producer as assisted by dot-blot assay and in vitro solution assays. The in vitro interactions of these proteases with host plant PIs were studied by reverse zymography and gel X-ray contact print (GXCP) analysis. Reduction in activity of PIs and degradation pattern of PI bands on gels in presence of trypsin and protease extract of Bacillus sp. YP1 indicates inactivation of PIs. Thus, conserved nature and in vitro response to PI proteins advocates role of gut bacterial flora in H. armigera digestive physiology.

Original languageEnglish
JournalJournal of the Saudi Society of Agricultural Sciences
DOIs
StateAccepted/In press - 2018 Jan 1

Fingerprint

Digestive System Physiological Phenomena
digestive physiology
Bacillus (bacteria)
Helicoverpa armigera
Bacillus
host plants
digestive system
flora
bacterial communities
Proteins
proteins
proteinases
Peptide Hydrolases
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
gels
Gels
Cajanus
Bacillus megaterium
Agricultural Crops

Keywords

  • Gut bacterial proteases
  • H. armigera
  • Host plant defense
  • PIs
  • TRFLP

Cite this

@article{c523ea57c2cc4ea0b2006c5b36d46d88,
title = "Conserved nature of Helicoverpa armigera gut bacterial flora on different host plants and in vitro interactions with PI proteins advocates role in host digestive physiology",
abstract = "Helicoverpa armigera is anxious insect pest of agricultural crops. Array of defensive molecules in host plants and extensive use of chemical insecticides are unable to cease the attack incidences. Gut bacterial communities are found to contribute in various physiological activities in most of the arthropods. In the current study the bacterial communities were isolated from gut of H. armigera feeding on three host plants (Pigeonpea, Chickpea and Cotton) by culture dependent and culture independent methods. Predominant bacterial communities were identified by terminal restriction fragment length polymorphism (TRFLP). Three dominant phylotypes namely proteobacteria, actinobacteria and firmicutes were identified by TRFLP and found to conserve on different host plant selected. Five Bacillus species namely Bacillus sp. JR14, Bacillus sp. YP1, Bacillus safensis CG1, Bacillus subtillis KAVK2 and Bacillus megaterium 47N were purified by culture dependent method and identified by 16S rRNA sequencing. Among all identified Bacillus, Bacillus sp. YP1 strain was found to be potent protease producer as assisted by dot-blot assay and in vitro solution assays. The in vitro interactions of these proteases with host plant PIs were studied by reverse zymography and gel X-ray contact print (GXCP) analysis. Reduction in activity of PIs and degradation pattern of PI bands on gels in presence of trypsin and protease extract of Bacillus sp. YP1 indicates inactivation of PIs. Thus, conserved nature and in vitro response to PI proteins advocates role of gut bacterial flora in H. armigera digestive physiology.",
keywords = "Gut bacterial proteases, H. armigera, Host plant defense, PIs, TRFLP",
author = "Shinde, {Ashok A.} and Shaikh, {Faiyaz K.} and Gadge, {Prafull P.} and Padul, {Manohar V.} and Govindwar, {Sanjay Prabhu} and Kachole, {Manvendra S.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.jssas.2017.03.004",
language = "English",
journal = "Journal of the Saudi Society of Agricultural Sciences",
issn = "1658-077X",

}

Conserved nature of Helicoverpa armigera gut bacterial flora on different host plants and in vitro interactions with PI proteins advocates role in host digestive physiology. / Shinde, Ashok A.; Shaikh, Faiyaz K.; Gadge, Prafull P.; Padul, Manohar V.; Govindwar, Sanjay Prabhu; Kachole, Manvendra S.

In: Journal of the Saudi Society of Agricultural Sciences, 01.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Conserved nature of Helicoverpa armigera gut bacterial flora on different host plants and in vitro interactions with PI proteins advocates role in host digestive physiology

AU - Shinde, Ashok A.

AU - Shaikh, Faiyaz K.

AU - Gadge, Prafull P.

AU - Padul, Manohar V.

AU - Govindwar, Sanjay Prabhu

AU - Kachole, Manvendra S.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Helicoverpa armigera is anxious insect pest of agricultural crops. Array of defensive molecules in host plants and extensive use of chemical insecticides are unable to cease the attack incidences. Gut bacterial communities are found to contribute in various physiological activities in most of the arthropods. In the current study the bacterial communities were isolated from gut of H. armigera feeding on three host plants (Pigeonpea, Chickpea and Cotton) by culture dependent and culture independent methods. Predominant bacterial communities were identified by terminal restriction fragment length polymorphism (TRFLP). Three dominant phylotypes namely proteobacteria, actinobacteria and firmicutes were identified by TRFLP and found to conserve on different host plant selected. Five Bacillus species namely Bacillus sp. JR14, Bacillus sp. YP1, Bacillus safensis CG1, Bacillus subtillis KAVK2 and Bacillus megaterium 47N were purified by culture dependent method and identified by 16S rRNA sequencing. Among all identified Bacillus, Bacillus sp. YP1 strain was found to be potent protease producer as assisted by dot-blot assay and in vitro solution assays. The in vitro interactions of these proteases with host plant PIs were studied by reverse zymography and gel X-ray contact print (GXCP) analysis. Reduction in activity of PIs and degradation pattern of PI bands on gels in presence of trypsin and protease extract of Bacillus sp. YP1 indicates inactivation of PIs. Thus, conserved nature and in vitro response to PI proteins advocates role of gut bacterial flora in H. armigera digestive physiology.

AB - Helicoverpa armigera is anxious insect pest of agricultural crops. Array of defensive molecules in host plants and extensive use of chemical insecticides are unable to cease the attack incidences. Gut bacterial communities are found to contribute in various physiological activities in most of the arthropods. In the current study the bacterial communities were isolated from gut of H. armigera feeding on three host plants (Pigeonpea, Chickpea and Cotton) by culture dependent and culture independent methods. Predominant bacterial communities were identified by terminal restriction fragment length polymorphism (TRFLP). Three dominant phylotypes namely proteobacteria, actinobacteria and firmicutes were identified by TRFLP and found to conserve on different host plant selected. Five Bacillus species namely Bacillus sp. JR14, Bacillus sp. YP1, Bacillus safensis CG1, Bacillus subtillis KAVK2 and Bacillus megaterium 47N were purified by culture dependent method and identified by 16S rRNA sequencing. Among all identified Bacillus, Bacillus sp. YP1 strain was found to be potent protease producer as assisted by dot-blot assay and in vitro solution assays. The in vitro interactions of these proteases with host plant PIs were studied by reverse zymography and gel X-ray contact print (GXCP) analysis. Reduction in activity of PIs and degradation pattern of PI bands on gels in presence of trypsin and protease extract of Bacillus sp. YP1 indicates inactivation of PIs. Thus, conserved nature and in vitro response to PI proteins advocates role of gut bacterial flora in H. armigera digestive physiology.

KW - Gut bacterial proteases

KW - H. armigera

KW - Host plant defense

KW - PIs

KW - TRFLP

UR - http://www.scopus.com/inward/record.url?scp=85029394099&partnerID=8YFLogxK

U2 - 10.1016/j.jssas.2017.03.004

DO - 10.1016/j.jssas.2017.03.004

M3 - Article

AN - SCOPUS:85029394099

JO - Journal of the Saudi Society of Agricultural Sciences

JF - Journal of the Saudi Society of Agricultural Sciences

SN - 1658-077X

ER -