Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique

D. Y. Min, M. H. Leem, J. M. Kim, Y. K. Choi

Research output: Contribution to journalArticle

Abstract

Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12-170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with different mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum (HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 94 kDa on EITB may connote the important significance on immune response in T. vaginalis infection.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalKisaengch'unghak chapchi. The Korean journal of parasitology
Volume30
Issue number4
DOIs
StatePublished - 1992 Dec

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