Characterization of CeHDA-7, a class II histone deacetylase interacting with MEF-2 in Caenorhabditis elegans

Kyu Yeong Choi, Yon Ju Ji, Changhoon Jee, Do Han Kim, Joohong Ahnn

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

As a result of screen searching for proteins interacting with MEF-2 transcription factor, we have identified the hda-7 gene in Caenorhabditis elegans. The hda-7 locus encodes a class II histone deacetylase containing a highly conserved catalytic domain. C. elegans HDA-7 protein translated in vitro demonstrated a direct interaction with CeMEF-2, as shown in other organisms. CeHDA-7 is abundantly expressed in body-wall muscle cells, neurons, and hypodermal seam cells, similar to CeMEF-2 expression patterns. Consistent with previously known phenotypes observed in mef-2 deletion mutants [Dev. Biol. 223 (2000) 431], RNA interference targeted for hda-7 did not result in muscle function or developmental defects.

Original languageEnglish
Pages (from-to)1295-1300
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number4
DOIs
StatePublished - 2002 Jul 4

Fingerprint

Histone Deacetylases
Caenorhabditis elegans
Muscle
RNA Interference
Muscle Cells
Neurons
Catalytic Domain
Proteins
Transcription Factors
Genes
Cells
RNA
Phenotype
Muscles
Defects
In Vitro Techniques

Keywords

  • C. elegans
  • HDAC
  • Histone deacetylase
  • MEF-2
  • hda-7

Cite this

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abstract = "As a result of screen searching for proteins interacting with MEF-2 transcription factor, we have identified the hda-7 gene in Caenorhabditis elegans. The hda-7 locus encodes a class II histone deacetylase containing a highly conserved catalytic domain. C. elegans HDA-7 protein translated in vitro demonstrated a direct interaction with CeMEF-2, as shown in other organisms. CeHDA-7 is abundantly expressed in body-wall muscle cells, neurons, and hypodermal seam cells, similar to CeMEF-2 expression patterns. Consistent with previously known phenotypes observed in mef-2 deletion mutants [Dev. Biol. 223 (2000) 431], RNA interference targeted for hda-7 did not result in muscle function or developmental defects.",
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Characterization of CeHDA-7, a class II histone deacetylase interacting with MEF-2 in Caenorhabditis elegans. / Yeong Choi, Kyu; Ju Ji, Yon; Jee, Changhoon; Han Kim, Do; Ahnn, Joohong.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 4, 04.07.2002, p. 1295-1300.

Research output: Contribution to journalArticle

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AU - Han Kim, Do

AU - Ahnn, Joohong

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