Characterization of a nuclear factor that binds to AP1-like element in the rat p53 promoter during liver regeneration

Minhyung Lee, Sunhee Yu, Jong Sang Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The transcription level of the rat p53 gene increases at 5-12 h in the regenerating liver after partial hepatectomy. It was previously reported that an activator protein 1 (AP1)-like element (-264 - 284) mediated the induced transcription of the rat p53 gene during liver regeneration. In this study, we characterize the protein binding to the AP1-like element by various methods. Oligonucleotide competition assays showed that the binding protein did not require AP1 consensus sequence. Therefore, the binding protein is not an AP1 family protein. Zn2+ was required for maximum DNA-binding activity of the protein, suggesting that the binding protein contains zinc fingers. The binding protein was highly resistant to denaturant. Even 1.8 M urea did not eliminate the protein-DNA complexes. In addition, the binding protein was stable up to 55°C. The protein-DNA complexes were abolished in the presence of 0.6 M NaCl and higher. Protease clipping assay showed that the protein had a protease-resistant core DNA binding domain. These results provided new insights into the structure of the protein that binds to the AP1-like element of the p53 promoter during liver regeneration. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)124-132
Number of pages9
JournalJournal of Cellular Biochemistry
Volume80
Issue number1
DOIs
StatePublished - 2000 Nov 20

Fingerprint

Liver Regeneration
Transcription Factor AP-1
Liver
Rats
Carrier Proteins
p53 Genes
Proteins
Protein Binding
DNA
Transcription
Peptide Hydrolases
Assays
Genes
Zinc Fingers
Consensus Sequence
DNA-Binding Proteins
Hepatectomy
Oligonucleotides
Urea
Zinc

Keywords

  • AP1-like element
  • Characterization
  • Liver regeneration
  • P53 promoter
  • Partial hepatectomy
  • Transcription regulation
  • Zinc finger

Cite this

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abstract = "The transcription level of the rat p53 gene increases at 5-12 h in the regenerating liver after partial hepatectomy. It was previously reported that an activator protein 1 (AP1)-like element (-264 - 284) mediated the induced transcription of the rat p53 gene during liver regeneration. In this study, we characterize the protein binding to the AP1-like element by various methods. Oligonucleotide competition assays showed that the binding protein did not require AP1 consensus sequence. Therefore, the binding protein is not an AP1 family protein. Zn2+ was required for maximum DNA-binding activity of the protein, suggesting that the binding protein contains zinc fingers. The binding protein was highly resistant to denaturant. Even 1.8 M urea did not eliminate the protein-DNA complexes. In addition, the binding protein was stable up to 55°C. The protein-DNA complexes were abolished in the presence of 0.6 M NaCl and higher. Protease clipping assay showed that the protein had a protease-resistant core DNA binding domain. These results provided new insights into the structure of the protein that binds to the AP1-like element of the p53 promoter during liver regeneration. (C) 2000 Wiley-Liss, Inc.",
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Characterization of a nuclear factor that binds to AP1-like element in the rat p53 promoter during liver regeneration. / Lee, Minhyung; Yu, Sunhee; Park, Jong Sang.

In: Journal of Cellular Biochemistry, Vol. 80, No. 1, 20.11.2000, p. 124-132.

Research output: Contribution to journalArticle

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