Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

Yunmee Lho, Carel W. le Roux, Hyeon Soo Park, Gon Sup Kim, Jeeyoun Jung, Geum Sook Hwang, Youn Kyoung Seo, Tae Kyung Ha, Eunyoung Ha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. Methods: Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. Results: Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. Conclusion: This study proves that VSG suppresses hepatic glucose production.

Original languageEnglish
Pages (from-to)2002-2010
Number of pages9
JournalObesity Surgery
Volume25
Issue number11
DOIs
StatePublished - 2015 Nov 1

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Gastrectomy
Glucose
Triiodothyronine
Blood Glucose
Liver
Eating
Body Weight
Insulin
Glucose-6-Phosphatase
Phosphoenolpyruvate
Metabolomics
Adenosine Monophosphate
Serum
Pyruvic Acid
Thyroxine
Thyroid Hormones
NADP
NAD
Proteomics
Fluorescent Antibody Technique

Keywords

  • Gluconeogenesis
  • Metabolome
  • Proteome
  • Vertical sleeve gastrectomy

Cite this

Lho, Y., le Roux, C. W., Park, H. S., Kim, G. S., Jung, J., Hwang, G. S., ... Ha, E. (2015). Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy. Obesity Surgery, 25(11), 2002-2010. https://doi.org/10.1007/s11695-015-1636-4
Lho, Yunmee ; le Roux, Carel W. ; Park, Hyeon Soo ; Kim, Gon Sup ; Jung, Jeeyoun ; Hwang, Geum Sook ; Seo, Youn Kyoung ; Ha, Tae Kyung ; Ha, Eunyoung. / Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy. In: Obesity Surgery. 2015 ; Vol. 25, No. 11. pp. 2002-2010.
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Lho, Y, le Roux, CW, Park, HS, Kim, GS, Jung, J, Hwang, GS, Seo, YK, Ha, TK & Ha, E 2015, 'Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy', Obesity Surgery, vol. 25, no. 11, pp. 2002-2010. https://doi.org/10.1007/s11695-015-1636-4

Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy. / Lho, Yunmee; le Roux, Carel W.; Park, Hyeon Soo; Kim, Gon Sup; Jung, Jeeyoun; Hwang, Geum Sook; Seo, Youn Kyoung; Ha, Tae Kyung; Ha, Eunyoung.

In: Obesity Surgery, Vol. 25, No. 11, 01.11.2015, p. 2002-2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

AU - Lho, Yunmee

AU - le Roux, Carel W.

AU - Park, Hyeon Soo

AU - Kim, Gon Sup

AU - Jung, Jeeyoun

AU - Hwang, Geum Sook

AU - Seo, Youn Kyoung

AU - Ha, Tae Kyung

AU - Ha, Eunyoung

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Background: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. Methods: Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. Results: Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. Conclusion: This study proves that VSG suppresses hepatic glucose production.

AB - Background: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. Methods: Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. Results: Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. Conclusion: This study proves that VSG suppresses hepatic glucose production.

KW - Gluconeogenesis

KW - Metabolome

KW - Proteome

KW - Vertical sleeve gastrectomy

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Lho Y, le Roux CW, Park HS, Kim GS, Jung J, Hwang GS et al. Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy. Obesity Surgery. 2015 Nov 1;25(11):2002-2010. https://doi.org/10.1007/s11695-015-1636-4