Cell sheet detachment from poly(N-vinylcaprolactam-co-N- isopropylacrylamide) grafted onto tissue culture polystyrene dishes

Youn Mook Lim, Joon Pyo Jeun, Joon Ho Lee, Young Moo Lee, Young Chang Nho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fabrication of functional tissue constructs using sandwiched layers of cultured cells could prove to be an attractive approach to tissue engineering. Rapid detachment of cultured cell sheets is a very important recovery method that permits facile manipulation of the sheet and prevents functional damage. To induce hydrophilic and hydrophobic structural changes on the required culture substrate in response to a culture temperature alteration, poly(N- vinylcaprolactam-co-N-isopropylacrylamide) (PNVCL-co-PNIPAAm) was grafted onto tissue culture polystyrene (TCPS) dishes through electron beam irradiation. Chemical analyses using ATR-FTIR spectroscopy revealed that PNVCL-co-PNIPAAm was successfully grafted onto the surfaces of the TCPS dishes. The TCPS dishes grafted with PNVCL-co-PNIPAAm were utilized for the cell sheet detachment experiments. Approximately 150 min was required to detach most of the cell sheets from the PNVCL- co-PNIPAAm-grafted TCPS surfaces.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume13
Issue number1
StatePublished - 2007 Jan 1

Fingerprint

Tissue culture
Polystyrenes
Cells
Tissue engineering
Electron beams
Irradiation
Spectroscopy
Tissue
Fabrication
Recovery
N-isopropylacrylamide
poly-N-isopropylacrylamide
poly-N-vinylcaprolactam
Substrates
Experiments
Temperature

Keywords

  • Cell culture
  • Cell detachment
  • Human fibroblast
  • Hydration
  • Poly(N-isopropylacrylamide)
  • Poly(N-vinylcaprolactam)

Cite this

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title = "Cell sheet detachment from poly(N-vinylcaprolactam-co-N- isopropylacrylamide) grafted onto tissue culture polystyrene dishes",
abstract = "Fabrication of functional tissue constructs using sandwiched layers of cultured cells could prove to be an attractive approach to tissue engineering. Rapid detachment of cultured cell sheets is a very important recovery method that permits facile manipulation of the sheet and prevents functional damage. To induce hydrophilic and hydrophobic structural changes on the required culture substrate in response to a culture temperature alteration, poly(N- vinylcaprolactam-co-N-isopropylacrylamide) (PNVCL-co-PNIPAAm) was grafted onto tissue culture polystyrene (TCPS) dishes through electron beam irradiation. Chemical analyses using ATR-FTIR spectroscopy revealed that PNVCL-co-PNIPAAm was successfully grafted onto the surfaces of the TCPS dishes. The TCPS dishes grafted with PNVCL-co-PNIPAAm were utilized for the cell sheet detachment experiments. Approximately 150 min was required to detach most of the cell sheets from the PNVCL- co-PNIPAAm-grafted TCPS surfaces.",
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Cell sheet detachment from poly(N-vinylcaprolactam-co-N- isopropylacrylamide) grafted onto tissue culture polystyrene dishes. / Lim, Youn Mook; Jeun, Joon Pyo; Lee, Joon Ho; Lee, Young Moo; Nho, Young Chang.

In: Journal of Industrial and Engineering Chemistry, Vol. 13, No. 1, 01.01.2007, p. 21-26.

Research output: Contribution to journalArticle

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AU - Lim, Youn Mook

AU - Jeun, Joon Pyo

AU - Lee, Joon Ho

AU - Lee, Young Moo

AU - Nho, Young Chang

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AB - Fabrication of functional tissue constructs using sandwiched layers of cultured cells could prove to be an attractive approach to tissue engineering. Rapid detachment of cultured cell sheets is a very important recovery method that permits facile manipulation of the sheet and prevents functional damage. To induce hydrophilic and hydrophobic structural changes on the required culture substrate in response to a culture temperature alteration, poly(N- vinylcaprolactam-co-N-isopropylacrylamide) (PNVCL-co-PNIPAAm) was grafted onto tissue culture polystyrene (TCPS) dishes through electron beam irradiation. Chemical analyses using ATR-FTIR spectroscopy revealed that PNVCL-co-PNIPAAm was successfully grafted onto the surfaces of the TCPS dishes. The TCPS dishes grafted with PNVCL-co-PNIPAAm were utilized for the cell sheet detachment experiments. Approximately 150 min was required to detach most of the cell sheets from the PNVCL- co-PNIPAAm-grafted TCPS surfaces.

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