CCAAT/enhancer-binding protein beta (C/EBPβ) is an important mediator of 1,25 dihydroxyvitamin D3 (1,25D3)-induced receptor activator of nuclear factor kappa-B ligand (RANKL) expression in osteoblasts

Sungsin Jo, Yun Young Lee, Jinil Han, Young Lim Lee, Subin Yoon, Jaehyun Lee, Younseo Oh, Joong Soo Han, Il Hoon Sung, Ye Soo Park, Tae Hwan Kim

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Receptor activator of nuclear factor kappa B ligand (RANKL) expression in osteoblasts is regulated by 1,25-dihydroxyvitamin D3 (1,25D3). CCAAT/enhancer-binding protein beta (C/EBPβ) has been proposed to function as a transcription factor and upregulate RANKL expression, but it is still uncertain how C/EBPβ is involved in 1,25D3-induced RANKL expression of osteoblasts. 1,25D3 stimulation increased the expression of RANKL and C/EPBβ genes in osteoblasts and enhanced phosphorylation and stability of these proteins. Moreover, induction of RANKL expression by 1,25D3 in osteoblasts was downregulated upon knockdown of C/EBPβ. In contrast, C/EBPβ overexpression directly upregulated RANKL promoter activity and exhibited a synergistic effect on 1,25D3-induced RANKL expression. In particular, 1,25D3 treatment of osteoblasts increased C/EBPβ protein binding to the RANKL promoter. In conclusion, C/EBPβ is required for induction of RANKL by 1,25D3.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalBMB reports
Volume52
Issue number6
DOIs
StatePublished - 2019

Keywords

  • 1,25-dihydroxyvitamin D3 (1,25D3)
  • C/EBPβ
  • Osteoblasts
  • RANKL

Fingerprint Dive into the research topics of 'CCAAT/enhancer-binding protein beta (C/EBPβ) is an important mediator of 1,25 dihydroxyvitamin D3 (1,25D3)-induced receptor activator of nuclear factor kappa-B ligand (RANKL) expression in osteoblasts'. Together they form a unique fingerprint.

Cite this