Calreticulin couples calcium release and calcium influx in integrin- mediated calcium signaling

Min Seong Kwon, Chun Shik Park, Kyeong Rock Choi, Chul Seung Park, Joohong Ahnn, Jae Il Kim, Soo Hyun Eom, Stephen J. Kaufman, Woo Keun Song

Research output: Contribution to journalArticle

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Abstract

The engagement of integrin α7 in E63 skeletal muscle cells by laminin or anti-α7 antibodies triggered transient elevations in the intracellular free Ca2+ concentration that resulted from both inositol triphosphate- evoked Ca2+ release from intracellular stores and extracellular Ca2+ influx through voltage-gated, L-type Ca2+ channels. The extracellular domain of integrin α7 was found to associate with both ectocalreticulin and dihydropyridine receptor on the cell surface. Calreticulin appears to also associate with cytoplasmic domain of integrin α7 in a manner highly dependent on the cytosolic Ca2+ concentration. It appeared that intracellular Ca2+ release was a prerequisite for Ca2+ reflux and that calreticulin associated with the integrin cytoplasmic domain mediated the coupling of between the Ca2+ release and Ca2+ influx. These findings suggest that calreticulin serves as a cytosolic activator of integrin and a signal transducer between integrins and Ca2+ channels on the cell surface.

Original languageEnglish
Pages (from-to)1433-1443
Number of pages11
JournalMolecular Biology of the Cell
Volume11
Issue number4
DOIs
StatePublished - 2000 Jan 1

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Calreticulin
Calcium Signaling
Integrins
Calcium
L-Type Calcium Channels
Laminin
Inositol
Transducers
Muscle Cells
Anti-Idiotypic Antibodies
Skeletal Muscle

Cite this

Kwon, Min Seong ; Park, Chun Shik ; Choi, Kyeong Rock ; Park, Chul Seung ; Ahnn, Joohong ; Kim, Jae Il ; Eom, Soo Hyun ; Kaufman, Stephen J. ; Song, Woo Keun. / Calreticulin couples calcium release and calcium influx in integrin- mediated calcium signaling. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 4. pp. 1433-1443.
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Kwon, MS, Park, CS, Choi, KR, Park, CS, Ahnn, J, Kim, JI, Eom, SH, Kaufman, SJ & Song, WK 2000, 'Calreticulin couples calcium release and calcium influx in integrin- mediated calcium signaling', Molecular Biology of the Cell, vol. 11, no. 4, pp. 1433-1443. https://doi.org/10.1091/mbc.11.4.1433

Calreticulin couples calcium release and calcium influx in integrin- mediated calcium signaling. / Kwon, Min Seong; Park, Chun Shik; Choi, Kyeong Rock; Park, Chul Seung; Ahnn, Joohong; Kim, Jae Il; Eom, Soo Hyun; Kaufman, Stephen J.; Song, Woo Keun.

In: Molecular Biology of the Cell, Vol. 11, No. 4, 01.01.2000, p. 1433-1443.

Research output: Contribution to journalArticle

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AU - Kim, Jae Il

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AU - Song, Woo Keun

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