Brevibacillus laterosporus MTCC 2298: A potential azo dye degrader

S. S. Gomare, S. P. Govindwar

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aims: To evaluate the potential of Brevibacillus laterosporus MTCC 2298 for the decolourization of different textile azo dyes including methyl red, mechanism of biotransformation and the toxicity of products. Methods and Results: Brevibacillus laterosporus showed decolourization of thirteen different azo dyes including methyl red. Decolourization of methyl red was faster (93% within 12 h) under static condition at the concentration 0·2 g l -1. Induction in the activities of lignin peroxidase, laccase, aminopyrine N-demethylase, NADH-DCIP reductase and malachite green reductase was observed in the cells obtained after decolourization. Fourier transform infra-red spectral analysis of products indicated conversion of methyl red into secondary aryl amines and nitrosamines, which further transformed into the aromatic nitro compounds. Gas chromatography-mass spectroscopy analysis suggested conversion of methyl red into high molecular weight complex derivatives. The heterocyclic substituted aryl amine (m/z 281), p-(N,N di formyl)-substituted para-di amino benzene derivative (m/z 355) and p-di-amino benzene derivative (m/z 282) are the mainly elected biotransformation products. Microbial and phytotoxicity studies suggested nontoxic nature of the biotransformation products. Conclusions: Brevibacillus laterosporus has potential for the decolourization of different textile azo dyes. Significance and Impact of the Study: Brevibacillus laterosporus decolourized different azo dyes including methyl red and can be utilized for textile dye decolourization.

Original languageEnglish
Pages (from-to)993-1004
Number of pages12
JournalJournal of Applied Microbiology
Volume106
Issue number3
DOIs
StatePublished - 2009 Mar 1

Fingerprint

Brevibacillus
Azo Compounds
Textiles
Biotransformation
Benzene Derivatives
Amines
Aminopyrine N-Demethylase
Nitro Compounds
Laccase
Nitrosamines
Fourier Analysis
Gas Chromatography
methyl red
Mass Spectrometry
Oxidoreductases
Coloring Agents
Molecular Weight

Keywords

  • Azo dyes
  • Brevibacillus laterosporus
  • GC-MS
  • Methyl red
  • Phytotoxicity

Cite this

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abstract = "Aims: To evaluate the potential of Brevibacillus laterosporus MTCC 2298 for the decolourization of different textile azo dyes including methyl red, mechanism of biotransformation and the toxicity of products. Methods and Results: Brevibacillus laterosporus showed decolourization of thirteen different azo dyes including methyl red. Decolourization of methyl red was faster (93{\%} within 12 h) under static condition at the concentration 0·2 g l -1. Induction in the activities of lignin peroxidase, laccase, aminopyrine N-demethylase, NADH-DCIP reductase and malachite green reductase was observed in the cells obtained after decolourization. Fourier transform infra-red spectral analysis of products indicated conversion of methyl red into secondary aryl amines and nitrosamines, which further transformed into the aromatic nitro compounds. Gas chromatography-mass spectroscopy analysis suggested conversion of methyl red into high molecular weight complex derivatives. The heterocyclic substituted aryl amine (m/z 281), p-(N,N di formyl)-substituted para-di amino benzene derivative (m/z 355) and p-di-amino benzene derivative (m/z 282) are the mainly elected biotransformation products. Microbial and phytotoxicity studies suggested nontoxic nature of the biotransformation products. Conclusions: Brevibacillus laterosporus has potential for the decolourization of different textile azo dyes. Significance and Impact of the Study: Brevibacillus laterosporus decolourized different azo dyes including methyl red and can be utilized for textile dye decolourization.",
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Brevibacillus laterosporus MTCC 2298 : A potential azo dye degrader. / Gomare, S. S.; Govindwar, S. P.

In: Journal of Applied Microbiology, Vol. 106, No. 3, 01.03.2009, p. 993-1004.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Brevibacillus laterosporus MTCC 2298

T2 - A potential azo dye degrader

AU - Gomare, S. S.

AU - Govindwar, S. P.

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AB - Aims: To evaluate the potential of Brevibacillus laterosporus MTCC 2298 for the decolourization of different textile azo dyes including methyl red, mechanism of biotransformation and the toxicity of products. Methods and Results: Brevibacillus laterosporus showed decolourization of thirteen different azo dyes including methyl red. Decolourization of methyl red was faster (93% within 12 h) under static condition at the concentration 0·2 g l -1. Induction in the activities of lignin peroxidase, laccase, aminopyrine N-demethylase, NADH-DCIP reductase and malachite green reductase was observed in the cells obtained after decolourization. Fourier transform infra-red spectral analysis of products indicated conversion of methyl red into secondary aryl amines and nitrosamines, which further transformed into the aromatic nitro compounds. Gas chromatography-mass spectroscopy analysis suggested conversion of methyl red into high molecular weight complex derivatives. The heterocyclic substituted aryl amine (m/z 281), p-(N,N di formyl)-substituted para-di amino benzene derivative (m/z 355) and p-di-amino benzene derivative (m/z 282) are the mainly elected biotransformation products. Microbial and phytotoxicity studies suggested nontoxic nature of the biotransformation products. Conclusions: Brevibacillus laterosporus has potential for the decolourization of different textile azo dyes. Significance and Impact of the Study: Brevibacillus laterosporus decolourized different azo dyes including methyl red and can be utilized for textile dye decolourization.

KW - Azo dyes

KW - Brevibacillus laterosporus

KW - GC-MS

KW - Methyl red

KW - Phytotoxicity

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JF - Journal of Applied Microbiology

SN - 1364-5072

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