Brassinosteroids are inherently biosynthesized in the primary roots of maize, Zea mays L.

Young Soo Kim, Tae-Wuk Kim, Seong Ki Kim

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

GC-MS analysis revealed that primary roots of maize contain 6-deoxocathasterone, 6-deoxoteasterone and 6-deoxotyphasterol. These brassinosteroids, and the previously identified campesterol, campestanol, 6-deoxocastasterone and castasterone, in the roots are members of a biosynthetic pathway to castasterone, namely the late C-6 oxidation pathway, suggesting that its biosynthetic pathway is operative in the roots. To verify this, a cell-free enzyme extract was prepared from maize roots, and enzymatic conversions from campesterol to castasterone through the aforementioned sterols and brassinosteroids were examined. The presence for the biosynthetic sequences, campesterol → 24-methylcholest-4-en-3β-ol → 24-methylcholest-4- en-3-one → 24-methylcholest-5 α-cholestan-3-one → campestanol and 6-deoxoteasterone → 6-deoxo-3-dehydroteasterone → 6-deoxotyphasterol → 6-deoxocastasterone → castasterone were demonstrated. These results indicate that maize roots contain a complete set of enzymes involved in the late C-6 oxidation pathway, thereby demonstrating that endogenous brassinosteroids are biosynthesized in the roots.

Original languageEnglish
Pages (from-to)1000-1006
Number of pages7
JournalPhytochemistry
Volume66
Issue number9
DOIs
StatePublished - 2005 May 1

Keywords

  • Biosynthesis
  • Brassinosteroids
  • Gamineae
  • Maize root
  • The late C-6 oxidation pathway
  • Zea mays L.

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