Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media

Vikash Bharti, Kumar Vikrant, Mandavi Goswami, Himanshu Tiwari, Ravi Kumar Sonwani, Jechan Lee, Daniel C.W. Tsang, Ki-Hyun Kim, Mohd Saeed, Sunil Kumar, Birendra Nath Rai, Balendu Shekher Giri, Ram Sharan Singh

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Bacterial species for metabolizing dye molecules were isolated from dye rich water bodies. The best microbial species for such an application was selected amongst the isolated bacterial populations by conducting methylene blue (MB) batch degradation studies with the bacterial strains using NaCl-yeast as a nutrient medium. The most suitable bacterial species was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process parameters were optimized and under the optimum conditions (e.g., inoculum size of 3 mL, temperature of 30 °C, 150 ppm, and time of 5 days), 96.2% of MB was removed. Furthermore, the effectiveness for the separation of MB combining bio-film with biochar was measured by a bio-sorption method in a packed bed bioreactor (PBBR) in which microbes was immobilized. The maximum MB removal efficiencies, when tested with 50 ppm dye using batch reactors containing free A. faecalis cells and the same cells immobilized on the biochar surface, were found to be 81.5% and 89.1%, respectively. The PBBR operated in continuous recycle mode at high dye concentration of 500 ppm provided 87.0% removal of MB through second-order kinetics over 10 days. The % removal was found in the order of PBBR>Immobilized batch>Free cell. The standalone biochar batch adsorption of MB can be described well by the pseudo-second order kinetics (R2 ≥ 0.978), indicating the major contribution of electron exchange-based valence forces in the sorption of MB onto the biochar surface. The Langmuir isotherm suggested a maximum monolayer adsorption capacity of 4.69 mg g−1 at 40 °C which was very close to experimentally calculated value (4.97 mg g−1). Moreover, the Casuarina seed biochar was reusable 5 times.

Original languageEnglish
Pages (from-to)356-364
Number of pages9
JournalEnvironmental Research
Volume171
DOIs
StatePublished - 2019 Apr 1

Fingerprint

Methylene Blue
Biodegradation
biodegradation
dye
Coloring Agents
bioreactor
Packed beds
Bioreactors
Alcaligenes faecalis
sorption
adsorption
kinetics
Adsorption
Sorption
yeast
biofilm
isotherm
Immobilized Cells
Kinetics
Body Water

Keywords

  • DNA sequencing
  • Dye removal
  • Engineered biochar
  • Immobilized bacterium
  • Pollution control

Cite this

Bharti, V., Vikrant, K., Goswami, M., Tiwari, H., Sonwani, R. K., Lee, J., ... Singh, R. S. (2019). Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media. Environmental Research, 171, 356-364. https://doi.org/10.1016/j.envres.2019.01.051
Bharti, Vikash ; Vikrant, Kumar ; Goswami, Mandavi ; Tiwari, Himanshu ; Sonwani, Ravi Kumar ; Lee, Jechan ; Tsang, Daniel C.W. ; Kim, Ki-Hyun ; Saeed, Mohd ; Kumar, Sunil ; Rai, Birendra Nath ; Giri, Balendu Shekher ; Singh, Ram Sharan. / Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media. In: Environmental Research. 2019 ; Vol. 171. pp. 356-364.
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title = "Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media",
abstract = "Bacterial species for metabolizing dye molecules were isolated from dye rich water bodies. The best microbial species for such an application was selected amongst the isolated bacterial populations by conducting methylene blue (MB) batch degradation studies with the bacterial strains using NaCl-yeast as a nutrient medium. The most suitable bacterial species was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process parameters were optimized and under the optimum conditions (e.g., inoculum size of 3 mL, temperature of 30 °C, 150 ppm, and time of 5 days), 96.2{\%} of MB was removed. Furthermore, the effectiveness for the separation of MB combining bio-film with biochar was measured by a bio-sorption method in a packed bed bioreactor (PBBR) in which microbes was immobilized. The maximum MB removal efficiencies, when tested with 50 ppm dye using batch reactors containing free A. faecalis cells and the same cells immobilized on the biochar surface, were found to be 81.5{\%} and 89.1{\%}, respectively. The PBBR operated in continuous recycle mode at high dye concentration of 500 ppm provided 87.0{\%} removal of MB through second-order kinetics over 10 days. The {\%} removal was found in the order of PBBR>Immobilized batch>Free cell. The standalone biochar batch adsorption of MB can be described well by the pseudo-second order kinetics (R2 ≥ 0.978), indicating the major contribution of electron exchange-based valence forces in the sorption of MB onto the biochar surface. The Langmuir isotherm suggested a maximum monolayer adsorption capacity of 4.69 mg g−1 at 40 °C which was very close to experimentally calculated value (4.97 mg g−1). Moreover, the Casuarina seed biochar was reusable 5 times.",
keywords = "DNA sequencing, Dye removal, Engineered biochar, Immobilized bacterium, Pollution control",
author = "Vikash Bharti and Kumar Vikrant and Mandavi Goswami and Himanshu Tiwari and Sonwani, {Ravi Kumar} and Jechan Lee and Tsang, {Daniel C.W.} and Ki-Hyun Kim and Mohd Saeed and Sunil Kumar and Rai, {Birendra Nath} and Giri, {Balendu Shekher} and Singh, {Ram Sharan}",
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Bharti, V, Vikrant, K, Goswami, M, Tiwari, H, Sonwani, RK, Lee, J, Tsang, DCW, Kim, K-H, Saeed, M, Kumar, S, Rai, BN, Giri, BS & Singh, RS 2019, 'Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media', Environmental Research, vol. 171, pp. 356-364. https://doi.org/10.1016/j.envres.2019.01.051

Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media. / Bharti, Vikash; Vikrant, Kumar; Goswami, Mandavi; Tiwari, Himanshu; Sonwani, Ravi Kumar; Lee, Jechan; Tsang, Daniel C.W.; Kim, Ki-Hyun; Saeed, Mohd; Kumar, Sunil; Rai, Birendra Nath; Giri, Balendu Shekher; Singh, Ram Sharan.

In: Environmental Research, Vol. 171, 01.04.2019, p. 356-364.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Biodegradation of methylene blue dye in a batch and continuous mode using biochar as packing media

AU - Bharti, Vikash

AU - Vikrant, Kumar

AU - Goswami, Mandavi

AU - Tiwari, Himanshu

AU - Sonwani, Ravi Kumar

AU - Lee, Jechan

AU - Tsang, Daniel C.W.

AU - Kim, Ki-Hyun

AU - Saeed, Mohd

AU - Kumar, Sunil

AU - Rai, Birendra Nath

AU - Giri, Balendu Shekher

AU - Singh, Ram Sharan

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Bacterial species for metabolizing dye molecules were isolated from dye rich water bodies. The best microbial species for such an application was selected amongst the isolated bacterial populations by conducting methylene blue (MB) batch degradation studies with the bacterial strains using NaCl-yeast as a nutrient medium. The most suitable bacterial species was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process parameters were optimized and under the optimum conditions (e.g., inoculum size of 3 mL, temperature of 30 °C, 150 ppm, and time of 5 days), 96.2% of MB was removed. Furthermore, the effectiveness for the separation of MB combining bio-film with biochar was measured by a bio-sorption method in a packed bed bioreactor (PBBR) in which microbes was immobilized. The maximum MB removal efficiencies, when tested with 50 ppm dye using batch reactors containing free A. faecalis cells and the same cells immobilized on the biochar surface, were found to be 81.5% and 89.1%, respectively. The PBBR operated in continuous recycle mode at high dye concentration of 500 ppm provided 87.0% removal of MB through second-order kinetics over 10 days. The % removal was found in the order of PBBR>Immobilized batch>Free cell. The standalone biochar batch adsorption of MB can be described well by the pseudo-second order kinetics (R2 ≥ 0.978), indicating the major contribution of electron exchange-based valence forces in the sorption of MB onto the biochar surface. The Langmuir isotherm suggested a maximum monolayer adsorption capacity of 4.69 mg g−1 at 40 °C which was very close to experimentally calculated value (4.97 mg g−1). Moreover, the Casuarina seed biochar was reusable 5 times.

AB - Bacterial species for metabolizing dye molecules were isolated from dye rich water bodies. The best microbial species for such an application was selected amongst the isolated bacterial populations by conducting methylene blue (MB) batch degradation studies with the bacterial strains using NaCl-yeast as a nutrient medium. The most suitable bacterial species was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process parameters were optimized and under the optimum conditions (e.g., inoculum size of 3 mL, temperature of 30 °C, 150 ppm, and time of 5 days), 96.2% of MB was removed. Furthermore, the effectiveness for the separation of MB combining bio-film with biochar was measured by a bio-sorption method in a packed bed bioreactor (PBBR) in which microbes was immobilized. The maximum MB removal efficiencies, when tested with 50 ppm dye using batch reactors containing free A. faecalis cells and the same cells immobilized on the biochar surface, were found to be 81.5% and 89.1%, respectively. The PBBR operated in continuous recycle mode at high dye concentration of 500 ppm provided 87.0% removal of MB through second-order kinetics over 10 days. The % removal was found in the order of PBBR>Immobilized batch>Free cell. The standalone biochar batch adsorption of MB can be described well by the pseudo-second order kinetics (R2 ≥ 0.978), indicating the major contribution of electron exchange-based valence forces in the sorption of MB onto the biochar surface. The Langmuir isotherm suggested a maximum monolayer adsorption capacity of 4.69 mg g−1 at 40 °C which was very close to experimentally calculated value (4.97 mg g−1). Moreover, the Casuarina seed biochar was reusable 5 times.

KW - DNA sequencing

KW - Dye removal

KW - Engineered biochar

KW - Immobilized bacterium

KW - Pollution control

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