Biodegradation of kerosene by Aspergillus ochraceus NCIM-1146

Ganesh Saratale, Satish Kalme, Sanjyot Bhosale, Sanjay Prabhu Govindwar

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The filamentous fungus Aspergillus ochraceus NCIM-1146 was found to degrade kerosene, when previously grown mycelium (96 h) was incubated in the broth containing kerosene. Higher levels of NADPH-DCIP reductase, aminopyrine N-demethylase and kerosene biodegradation activities were found to be present after the growth in potato dextrose broth for 96 h, when compared with the activities at different time intervals during the growth phase. NADPH was the preferred cofactor for enzyme activity, which was inhibited by CO, indicating cytochrome P450 mediated reactions. A significant increase in all the enzyme activities was observed when mycelium incubated for 18 h in mineral salts medium, containing cholesterol, camphor, naphthalene, 1,2-dimethoxybenzene, phenobarbital, n-hexane, kerosene or saffola oil as inducers. Acetaldehyde produced by alcohol dehydrogenase could be used as an indicator for the kerosene biodegradation.

Original languageEnglish
Pages (from-to)400-405
Number of pages6
JournalJournal of Basic Microbiology
Volume47
Issue number5
DOIs
StatePublished - 2007 Oct 31

Fingerprint

Aspergillus ochraceus
Kerosene
Mycelium
NADP
Aminopyrine N-Demethylase
Camphor
Acetaldehyde
Alcohol Dehydrogenase
Coenzymes
Carbon Monoxide
Phenobarbital
Growth
Solanum tuberosum
Cytochrome P-450 Enzyme System
Minerals
Oils
Fungi
Salts
Cholesterol
Glucose

Keywords

  • Amino N-demethylase
  • Aspergillus ochraceus
  • Biodegradation
  • Kerosene

Cite this

Saratale, Ganesh ; Kalme, Satish ; Bhosale, Sanjyot ; Govindwar, Sanjay Prabhu. / Biodegradation of kerosene by Aspergillus ochraceus NCIM-1146. In: Journal of Basic Microbiology. 2007 ; Vol. 47, No. 5. pp. 400-405.
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Biodegradation of kerosene by Aspergillus ochraceus NCIM-1146. / Saratale, Ganesh; Kalme, Satish; Bhosale, Sanjyot; Govindwar, Sanjay Prabhu.

In: Journal of Basic Microbiology, Vol. 47, No. 5, 31.10.2007, p. 400-405.

Research output: Contribution to journalArticle

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