Bio-inspired immobilization of cell-adhesive ligands on electrospun nanofibrous patches for cell delivery

Young Min Shin, Indong Jun, Youn Mook Lim, Taiyoun Rhim, Heungsoo Shin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

An electrospun fibrous mesh functionalized with a cell-adhesive peptide inspired by polydopamine coating is developed as a novel cardiac patch. The amount of polydopamine on the PLCL meshes is affected by several parameters such as pH, concentration, reaction time, and temperature. Immobilization of RGD peptide is then easily achieved on the polydopamine-coated meshes. The RGD-peptide-immobilized meshes increase adhesion of the myoblasts under serum-free conditions, and also significantly up-regulate their proliferation and the formation of multi-nucleated myotubes. Therefore, RGD-peptide- functionalized, electrospun cardiac patches prepared by polydopamine coating method can be a useful tool to replace damaged myocardium. Electrospun poly(L-lactide-co-caprolactone) fibers functionalized with a cell-adhesive peptide inspired by polydopamine coating are presented. The successful surface coating modulates adhesion, proliferation, and formation of multi-nucleated myotubes of myoblasts, which can be a useful tool as a novel cardiac patch to deliver therapeutic cell sources.

Original languageEnglish
Pages (from-to)555-564
Number of pages10
JournalMacromolecular Materials and Engineering
Volume298
Issue number5
DOIs
StatePublished - 2013 May 1

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Keywords

  • bio-inspired coatings
  • biomimetic
  • heart regeneration
  • nanofibrous patches
  • polydopamine

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