Berberine ameliorates brain inflammation in poloxamer 407-induced hyperlipidemic rats

Mia Kim, Tae Woon Kim, Chang Ju Kim, Mal Soon Shin, Minha Hong, Hye Sang Park, Sang Seo Park

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Purpose: Hyperlipidemia, which promotes the development of atherosclerosis, ischemic stroke, and other forms of brain injury, can be induced by poloxamer-407. Berberine is a primary pharmacological active component of Coptidis Rhizoma that has a number of therapeutic activities. This study investigated the effects of berberine on poloxamer-407-induced brain inflammation by evaluating its effects on short-term memory, cell proliferation, inflammation, and apoptosis in the hippocampus. Methods: To induce hyperlipidemia in a rat model, 500 mg/kg of poloxamer-407 was injected intraperitoneally. Berberine was orally administered to the rats in the berberine-treated groups once a day for 4 weeks. The step-down task avoidance task was performed to measure short-term memory. An analysis of serum lipids, immunohistochemistry for 5-bromo-2′-deoxyuri-dine, glial fibrillary acidic protein (GFAP), and ionized calcium-binding adapter molecule 1 (Iba1) in the dentate gyrus, and western blot analysis for Bax, Bcl-2, and cytochrome c in the hippocampus were performed. Results: In hyperlipidemic rats, berberine reduced the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol and increased the level of high-density lipoprotein cholesterol in hyperlipidemic rats. Berberine also increased cell proliferation and short-term memory, as well as decreasing the expression of GFAP, Iba1, Bax, and cytochrome c and increasing Bcl-2 expression. Conclusions: Berberine treatment improved short-term memory in hyperlipidemia by increasing neuronal proliferation and inhibiting neuronal apoptosis. Berberine treatment also improved lipid metabolism.

Original languageEnglish
Pages (from-to)S102-S110
JournalInternational Neurourology Journal
Volume23
DOIs
StatePublished - 2019 Jan 1

Keywords

  • Apoptosis
  • Berberine
  • Glial fibrillary acidic protein
  • Hyperlipidemia
  • Iba1
  • Poloxamer

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