Atmospheric pressure plasma irradiation facilitates transdermal permeability of aniline blue on porcine skin and the cellular permeability of keratinocytes with the production of nitric oxide

Sunmi Lee, Jongbong Choi, Junghyun Kim, Yongwoo Jang, Tae Ho Lim

Research output: Contribution to journalArticlepeer-review

Abstract

The transdermal delivery system of nutrients, cosmetics, and drugs is particularly at-tractive for painless, noninvasive delivery and sustainable release. Recently, atmospheric pressure plasma techniques have been of great interest to improve the drug absorption rate in transdermal delivery. Currently, plasma-mediated changes in the lipid composition of the stratum corneum are considered a possible mechanism to increase transdermal permeability. Nevertheless, its molecular and cellular mechanisms in transdermal delivery have been largely confined and still veiled. Herein, we present the effects of cold plasma on transdermal transmission on porcine skin and the cellular permeability of keratinocytes and further demonstrate the production of nitric oxide from keratinocytes. Consequently, argon plasma irradiation for 60 s resulted in 2.5-fold higher transdermal absorption of aniline blue dye on porcine skin compared to the nontreated control. In addition, the plasma-treated keratinocytes showed an increased transmission of high-molecular-weight molecules (70 and 150 kDa) with the production of nitric oxide. Therefore, these findings suggest a promot-ing effect of low-temperature plasma on transdermal absorption, even for high-molecular-weight molecules. Moreover, plasma-induced nitric oxide from keratinocytes is likely to regulate transdermal permeability in the epidermal layer.

Original languageEnglish
Article number2390
JournalApplied Sciences (Switzerland)
Volume11
Issue number5
DOIs
StatePublished - 2021 Mar 1

Keywords

  • Atmospheric pressure plasma
  • Nitric oxide
  • Plasma medicine
  • Transdermal delivery
  • Transdermal permeability

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