Antimelanogenic Efficacy of Melasolv (3,4,5-Trimethoxycinnamate Thymol Ester) in Melanocytes and Three-Dimensional Human Skin Equivalent

John Hwan Lee, Eun Soo Lee, Il Hong Bae, Jeong Ah Hwang, Se Hwa Kim, Dae Yong Kim, Nok Hyun Park, Ho Sik Rho, Yong Jin Kim, Seong Geun Oh, Chang Seok Lee

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background/Aims: Excessive melanogenesis often causes unaesthetic hyperpigmentation. In a previous report, our group introduced a newly synthesized depigmentary agent, Melasolv™ (3,4,5-trimethoxycinnamate thymol ester). In this study, we demonstrated the significant whitening efficacy of Melasolv using various melanocytes and human skin equivalents as in vitro experimental systems. Methods: The depigmentary effect of Melasolv was tested in melan-a cells (immortalized normal murine melanocytes), α-melanocyte-stimulating hormone (α-MSH)-stimulated B16 murine melanoma cells, primary normal human melanocytes (NHMs), and human skin equivalent (MelanoDerm). The whitening efficacy of Melasolv was further demonstrated by photography, time-lapse microscopy, Fontana-Masson (F&M) staining, and 2-photon microscopy. Results: Melasolv significantly inhibited melanogenesis in the melan-a and α-MSH-stimulated B16 cells. In human systems, Melasolv also clearly showed a whitening effect in NHMs and human skin equivalent, reflecting a decrease in melanin content. F&M staining and 2-photon microscopy revealed that Melasolv suppressed melanin transfer into multiple epidermal layers from melanocytes as well as melanin synthesis in human skin equivalent. Conclusion: Our study showed that Melasolv clearly exerts a whitening effect on various melanocytes and human skin equivalent. These results suggest the possibility that Melasolv can be used as a depigmentary agent to treat pigmentary disorders as well as an active ingredient in cosmetics to increase whitening efficacy.

Original languageEnglish
Pages (from-to)190-196
Number of pages7
JournalSkin Pharmacology and Physiology
Volume30
Issue number4
DOIs
StatePublished - 2017 Aug 1

Keywords

  • Depigmentary agent
  • Melanogenesis
  • Melasolv
  • Three-dimensional human skin equivalent

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    Lee, J. H., Lee, E. S., Bae, I. H., Hwang, J. A., Kim, S. H., Kim, D. Y., Park, N. H., Rho, H. S., Kim, Y. J., Oh, S. G., & Lee, C. S. (2017). Antimelanogenic Efficacy of Melasolv (3,4,5-Trimethoxycinnamate Thymol Ester) in Melanocytes and Three-Dimensional Human Skin Equivalent. Skin Pharmacology and Physiology, 30(4), 190-196. https://doi.org/10.1159/000477356