Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system

Changman Kim, Mi Yeon Kim, Iain Michie, Byong-Hun Jeon, Giuliano C. Premier, Sunghoon Park, Jung Rae Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results: In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions: Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes.

Original languageEnglish
Article number199
JournalBiotechnology for Biofuels
Volume10
Issue number1
DOIs
StatePublished - 2017 Aug 17

Fingerprint

pneumonia
Klebsiella pneumoniae
Glycerol
Fermentation
fermentation
Acids
acid
NAD
Coenzymes
Aldehyde Dehydrogenase
Electrodes
aldehyde
Aldehydes
electrode
Electrons
electron
Bioconversion
Biocatalysts
anoxic conditions
Oxidation-Reduction

Keywords

  • 3-hydroxypropionic acid
  • Bioelectrochemical system
  • Electro-fermentation
  • Klebsiella pneumoniae L17

Cite this

Kim, Changman ; Kim, Mi Yeon ; Michie, Iain ; Jeon, Byong-Hun ; Premier, Giuliano C. ; Park, Sunghoon ; Kim, Jung Rae. / Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system. In: Biotechnology for Biofuels. 2017 ; Vol. 10, No. 1.
@article{a37864da6db64559abbf76628752888e,
title = "Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system",
abstract = "Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results: In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions: Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes.",
keywords = "3-hydroxypropionic acid, Bioelectrochemical system, Electro-fermentation, Klebsiella pneumoniae L17",
author = "Changman Kim and Kim, {Mi Yeon} and Iain Michie and Byong-Hun Jeon and Premier, {Giuliano C.} and Sunghoon Park and Kim, {Jung Rae}",
year = "2017",
month = "8",
day = "17",
doi = "10.1186/s13068-017-0886-x",
language = "English",
volume = "10",
journal = "Biotechnology for Biofuels",
issn = "1754-6834",
number = "1",

}

Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system. / Kim, Changman; Kim, Mi Yeon; Michie, Iain; Jeon, Byong-Hun; Premier, Giuliano C.; Park, Sunghoon; Kim, Jung Rae.

In: Biotechnology for Biofuels, Vol. 10, No. 1, 199, 17.08.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system

AU - Kim, Changman

AU - Kim, Mi Yeon

AU - Michie, Iain

AU - Jeon, Byong-Hun

AU - Premier, Giuliano C.

AU - Park, Sunghoon

AU - Kim, Jung Rae

PY - 2017/8/17

Y1 - 2017/8/17

N2 - Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results: In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions: Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes.

AB - Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results: In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions: Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes.

KW - 3-hydroxypropionic acid

KW - Bioelectrochemical system

KW - Electro-fermentation

KW - Klebsiella pneumoniae L17

UR - http://www.scopus.com/inward/record.url?scp=85027890307&partnerID=8YFLogxK

U2 - 10.1186/s13068-017-0886-x

DO - 10.1186/s13068-017-0886-x

M3 - Article

AN - SCOPUS:85027890307

VL - 10

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

SN - 1754-6834

IS - 1

M1 - 199

ER -