An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection

Dongkyu Kang, Hyun Joo Ahn, Jiho Lee, Sang Kyung Kim, Jaechul Pyun, Chang Seon Song, Sang Jick Kim, Joonseok Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.

Original languageEnglish
Article number113369
JournalBiosensors and Bioelectronics
Volume190
DOIs
StatePublished - 2021 Oct 15

Keywords

  • Avian origin-virus
  • Homogeneous sandwich-immunoassay
  • Lanthanide-doped nanoparticles
  • Near-infrared region
  • Single-chain variable fragment

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