An FMN-containing NADH-quinone reductase from Streptomyces sp.

Hong Duk Youn, Jin-Won Lee, Hwan Youn, Jeong Kug Lee, Yung Chil Hah, Sa Ouk Kang

Research output: Contribution to journalArticle

Abstract

NADH-quinone reductase was purified 22-fold from the cytosolic fraction of Streptomyces sp. IMSNU-1 to apparent homogeneity, with an overall yield of 9%, by the purification procedure consisting of ammonium sulfate precipitation and DEAE Sephacel, Sephacryl S-200 and DEAE 5 PW chromatographies. The molecular mass of the enzyme determined by gel filtration chromatography was found to be 110 kDa. SDS-PAGE revealed that the enzyme consists of two subunits with a molecular mass of 54 kDa. The enzyme contained 1 mol of FMN per subunit as a cofactor. The A173/A457 ratio was 6.14 and the molar extinction coefficients were calculated to be 20, 800 and 25,400 M-1cm-1 at 349 and 457 nm, respectively. The N-terminal sequence of the enzyme contained the highly conserved fingerprint of ADP-binding domain. The enzyme used NADH as an electron donor and various quinones as electron acceptors. Cytochrome c was practically inactive. Air-stable flavin semiquinone was produced by the addition of NADH to the enzyme. Also, naphthosemiquinone was detected in the reaction mixture containing the enzyme.

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalJournal of Microbiology
Volume34
Issue number2
StatePublished - 1996 Jun 1

Fingerprint

NAD(P)H Dehydrogenase (Quinone)
Flavin Mononucleotide
Streptomyces
NAD
Enzymes
Electrons
Quinones
Ammonium Sulfate
Dermatoglyphics
Cytochromes c
Adenosine Diphosphate
Gel Chromatography
Chromatography
Polyacrylamide Gel Electrophoresis
Air

Keywords

  • FMN
  • NADH-quinone reductase
  • Semiquinone
  • Streptomyces sp

Cite this

Youn, H. D., Lee, J-W., Youn, H., Lee, J. K., Hah, Y. C., & Kang, S. O. (1996). An FMN-containing NADH-quinone reductase from Streptomyces sp. Journal of Microbiology, 34(2), 206-213.
Youn, Hong Duk ; Lee, Jin-Won ; Youn, Hwan ; Lee, Jeong Kug ; Hah, Yung Chil ; Kang, Sa Ouk. / An FMN-containing NADH-quinone reductase from Streptomyces sp. In: Journal of Microbiology. 1996 ; Vol. 34, No. 2. pp. 206-213.
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Youn, HD, Lee, J-W, Youn, H, Lee, JK, Hah, YC & Kang, SO 1996, 'An FMN-containing NADH-quinone reductase from Streptomyces sp.', Journal of Microbiology, vol. 34, no. 2, pp. 206-213.

An FMN-containing NADH-quinone reductase from Streptomyces sp. / Youn, Hong Duk; Lee, Jin-Won; Youn, Hwan; Lee, Jeong Kug; Hah, Yung Chil; Kang, Sa Ouk.

In: Journal of Microbiology, Vol. 34, No. 2, 01.06.1996, p. 206-213.

Research output: Contribution to journalArticle

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AU - Kang, Sa Ouk

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Youn HD, Lee J-W, Youn H, Lee JK, Hah YC, Kang SO. An FMN-containing NADH-quinone reductase from Streptomyces sp. Journal of Microbiology. 1996 Jun 1;34(2):206-213.