Allele-Specific phenotype suggests a possible stimulatory activity of RCAN-1 on calcineurin in caenorhabditis elegans

Weixun Li, Tae Woo Choi, Joohong Ahnn, Sun Kyung Lee

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Regulator of calcineurin 1 (RCAN1) binds to calcineurin through the PxIxIT motif, which is evolutionarily conserved. SP repeat phosphorylation in RCAN1 is required for its complete function. The specific interaction between RCAN1 and calcineurin is critical for calcium/calmodulin-dependent regulation of calcineurin serine/threonine phosphatase activity. In this study, we investigated two available deletion rcan-1 mutants in Caenorhabditis elegans, which proceed differently for transcription and translation. We found that rcan-1 may be required for calcineurin activity and possess calcineurin-independent function in body growth and egg-laying behavior. In the genetic background of enhanced calcineurin activity, the rcan-1 mutant expressing a truncated RCAN-1 which retains the calcineurin-binding PxIxIT motif but misses SP repeats stimulated growth, while rcan-1 lack mutant resulted in hyperactive egg-laying suppression. These data suggest rcan-1 has unknown functions independent of calcineurin, and may be a stimulatory calcineurin regulator under certain circumstances.

Original languageEnglish
Pages (from-to)827-833
Number of pages7
JournalMolecules and Cells
Volume39
Issue number11
DOIs
StatePublished - 2016 Nov 1

Fingerprint

Calcineurin
Caenorhabditis elegans
Alleles
Phenotype
Ovum
Phosphoprotein Phosphatases
Calmodulin
Growth
Phosphorylation

Keywords

  • C. elegans
  • Calcineurin
  • RCAN

Cite this

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title = "Allele-Specific phenotype suggests a possible stimulatory activity of RCAN-1 on calcineurin in caenorhabditis elegans",
abstract = "Regulator of calcineurin 1 (RCAN1) binds to calcineurin through the PxIxIT motif, which is evolutionarily conserved. SP repeat phosphorylation in RCAN1 is required for its complete function. The specific interaction between RCAN1 and calcineurin is critical for calcium/calmodulin-dependent regulation of calcineurin serine/threonine phosphatase activity. In this study, we investigated two available deletion rcan-1 mutants in Caenorhabditis elegans, which proceed differently for transcription and translation. We found that rcan-1 may be required for calcineurin activity and possess calcineurin-independent function in body growth and egg-laying behavior. In the genetic background of enhanced calcineurin activity, the rcan-1 mutant expressing a truncated RCAN-1 which retains the calcineurin-binding PxIxIT motif but misses SP repeats stimulated growth, while rcan-1 lack mutant resulted in hyperactive egg-laying suppression. These data suggest rcan-1 has unknown functions independent of calcineurin, and may be a stimulatory calcineurin regulator under certain circumstances.",
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Allele-Specific phenotype suggests a possible stimulatory activity of RCAN-1 on calcineurin in caenorhabditis elegans. / Li, Weixun; Choi, Tae Woo; Ahnn, Joohong; Lee, Sun Kyung.

In: Molecules and Cells, Vol. 39, No. 11, 01.11.2016, p. 827-833.

Research output: Contribution to journalArticleResearchpeer-review

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