TY - JOUR
T1 - AIEgen-based nanoprobe for the ATP sensing and imaging in cancer cells and embryonic stem cells
AU - Kim, Na Hee
AU - Kim, Byeong Wook
AU - Moon, Heechang
AU - Yoo, Hajung
AU - Kang, Rae Hyung
AU - Hur, Junho K.
AU - Oh, Yohan
AU - Kim, B. Moon
AU - Kim, Dokyoung
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - A turn-on fluorescent nanoprobe (named AAP-1), based on an aggregation-induced emission luminogen (AIEgen), is disclosed for the detection of adenosine triphosphate (ATP), which is an essential element in the biological system. Organic fluorophore (named TPE-TA) consists of tetraphenylethylene (TPE, sensing and signaling moiety) and mono-triamine (TA, sensing moiety), and it forms an aggregated form in aqueous media as a nanoprobe AAP-1. The nanoprobe AAP-1 has multiple electrostatic interactions as well as hydrophobic interactions with ATP, and it displays superior selectivity toward ATP, reliable sensitivity, with a detection limit around 0.275 ppb, and fast responsive (signal within 10 s). Such a fluorescent probe to monitor ATP has been actively pursued throughout fundamental and translational research areas. In vitro assay and a successful cellular ATP imaging application was demonstrated in cancer cells and embryonic stem cells. We expect that our work warrants further ATP-related studies throughout a variety of fields.
AB - A turn-on fluorescent nanoprobe (named AAP-1), based on an aggregation-induced emission luminogen (AIEgen), is disclosed for the detection of adenosine triphosphate (ATP), which is an essential element in the biological system. Organic fluorophore (named TPE-TA) consists of tetraphenylethylene (TPE, sensing and signaling moiety) and mono-triamine (TA, sensing moiety), and it forms an aggregated form in aqueous media as a nanoprobe AAP-1. The nanoprobe AAP-1 has multiple electrostatic interactions as well as hydrophobic interactions with ATP, and it displays superior selectivity toward ATP, reliable sensitivity, with a detection limit around 0.275 ppb, and fast responsive (signal within 10 s). Such a fluorescent probe to monitor ATP has been actively pursued throughout fundamental and translational research areas. In vitro assay and a successful cellular ATP imaging application was demonstrated in cancer cells and embryonic stem cells. We expect that our work warrants further ATP-related studies throughout a variety of fields.
KW - Adenosine triphosphate (ATP)
KW - Aggregation-induced emission (AIE)
KW - Cellular imaging
KW - Fluorescent probes
KW - Nanoprobe
UR - http://www.scopus.com/inward/record.url?scp=85100794618&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2021.338269
DO - 10.1016/j.aca.2021.338269
M3 - Article
C2 - 33648642
AN - SCOPUS:85100794618
VL - 1152
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
M1 - 338269
ER -