Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Sandeep Kumar, Shikha Jain, Neeraj Dilbaghi, Amrik Singh Ahluwalia, Ashraf Aly Hassan, Ki-Hyun Kim

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.

Original languageEnglish
Pages (from-to)190-213
Number of pages24
JournalTrends in Biochemical Sciences
Volume44
Issue number3
DOIs
StatePublished - 2019 Mar 1

Fingerprint

Catalytic DNA
Delivery of Health Care
Ligands
Catalyst activity
Personnel
Equipment and Supplies
Molecules

Cite this

Kumar, Sandeep ; Jain, Shikha ; Dilbaghi, Neeraj ; Ahluwalia, Amrik Singh ; Hassan, Ashraf Aly ; Kim, Ki-Hyun. / Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications. In: Trends in Biochemical Sciences. 2019 ; Vol. 44, No. 3. pp. 190-213.
@article{fda702416be84f2584c7c8a0719004e6,
title = "Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications",
abstract = "DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.",
author = "Sandeep Kumar and Shikha Jain and Neeraj Dilbaghi and Ahluwalia, {Amrik Singh} and Hassan, {Ashraf Aly} and Ki-Hyun Kim",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.tibs.2018.11.001",
language = "English",
volume = "44",
pages = "190--213",
journal = "Trends in Biochemical Sciences",
issn = "0968-0004",
number = "3",

}

Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications. / Kumar, Sandeep; Jain, Shikha; Dilbaghi, Neeraj; Ahluwalia, Amrik Singh; Hassan, Ashraf Aly; Kim, Ki-Hyun.

In: Trends in Biochemical Sciences, Vol. 44, No. 3, 01.03.2019, p. 190-213.

Research output: Contribution to journalReview articleResearchpeer-review

TY - JOUR

T1 - Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

AU - Kumar, Sandeep

AU - Jain, Shikha

AU - Dilbaghi, Neeraj

AU - Ahluwalia, Amrik Singh

AU - Hassan, Ashraf Aly

AU - Kim, Ki-Hyun

PY - 2019/3/1

Y1 - 2019/3/1

N2 - DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.

AB - DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.

UR - http://www.scopus.com/inward/record.url?scp=85058377173&partnerID=8YFLogxK

U2 - 10.1016/j.tibs.2018.11.001

DO - 10.1016/j.tibs.2018.11.001

M3 - Review article

VL - 44

SP - 190

EP - 213

JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

SN - 0968-0004

IS - 3

ER -