Adult rat odontoblasts lack noxious thermal sensitivity

K. Y. Yeon, G. Chung, M. S. Shin, S. J. Jung, J. S. Kim, S. B. Oh

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Dentin hypersensitivity is a common symptom treated in the dental clinic, yet the underlying cellular and molecular mechanisms are not clear. We hypothesized that odontoblasts detect noxious thermal stimuli by expressing temperature-sensing molecules, and investigated whether temperature-activated TRP channels (thermo-TRP channels), which are known to initiate temperature signaling, mediate temperature sensing in odontoblasts. mRNA expression of dentin sialophosphoprotein and collagenase type 1, odontoblast-specific proteins, was shown in acutely isolated adult rat odontoblasts by single-cell RT-PCR, while TRPV1, TRPV2, TRPM8, and TRPA1 were not detected. Application of noxious temperatures of 42°C and 12°C, as well as capsaicin, menthol, and icilin, agonists of thermo-TRP channels, failed to increase intracellular calcium concentration. Immunohistochemical study also revealed no expression of TRPV1. Thus, it is unlikely that odontoblasts serve as thermal sensors in teeth via thermo-TRP channels.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
JournalJournal of Dental Research
Volume88
Issue number4
DOIs
StatePublished - 2009 Aug 7

Keywords

  • Dentin hypersensitivity
  • Odontoblast
  • TRP

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