Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of angus steers between 9 and 16 months of age

S. B. Smith, G. W. Go, B. J. Johnson, K. Y. Chung, S. H. Choi, J. E. Sawyer, D. T. Silvey, L. A. Gilmore, G. Ghahramany, K. H. Kim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous adipose tissue contains the greatest concentration of MUFA and lowest concentration of SFA. Therefore, we hypothesized that brisket subcutaneous adipose tissue depots would exhibit greater adipogenic gene expression over time than other major subcutaneous adipose tissue depots. Four Angus steers, each at 9, 12, 14, and 16 mo of age, were harvested and fresh subcutaneous adipose tissue samples were collected from over the brisket, chuck, rib, loin, sirloin, round, flank, and plate. Relative gene expression for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β), stearoylcoenzyme A desaturase (SCD), AMP-activated protein kinase alpha (AMPKα), and G-coupled protein receptor 43 (GPR43) was analyzed by quantitative real-time PCR. Expression of C/EBPβ, PPARγ, and CPT-1β was greatest at 12 to 14 mo of age (all P < 0.0001) and declined to very low abundance by 16 mo of age in all depots. Expression of PPARγ and CPT-1β was greater (P < 0.03) in flank, rib, and sirloin subcutaneous adipose tissues than in brisket and round adipose tissues. The expression of the SCD gene did not differ among the 4 age groups (P = 0.95). The palmitoleic: stearic acid ratio (an estimate of SCD activity) was greater (P < 0.001) in the subcutaneous adipose tissues from brisket, plate, and round than in the loin, rib, and sirloin. Conversely, subcutaneous adipose tissue from the loin, rib, and sirloin had greater (P < 0.001) SCD gene expression than the brisket, plate, and round. In general, subcutaneous adipose tissues with the highest concentration of MUFA and least SFA consistently exhibited the least SCD gene expression and adipogenic gene expression. We conclude that MUFA in the brisket and other depots with large SCD indices were deposited before 9 mo of age, during a time when the subcutaneous adipocytes were highly differentiated.

Original languageEnglish
Pages (from-to)2505-2514
Number of pages10
JournalJournal of Animal Science
Volume90
Issue number8
DOIs
StatePublished - 2012 Aug 1

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Subcutaneous Fat
Angus
adipose tissue
Fatty Acids
fatty acid composition
Gene Expression
gene expression
Ribs
Carnitine O-Palmitoyltransferase
Peroxisome Proliferator-Activated Receptors
carnitine palmitoyltransferase
ribs
loins
Adipose Tissue
Cyclic GMP-Dependent Protein Kinases
AMP-Activated Protein Kinases
AMP-activated protein kinase
palmitoleic acid
gene overexpression
Adipocytes

Keywords

  • Adipose tissue
  • Bovine
  • Fatty acid
  • Gene expression
  • Growth

Cite this

Smith, S. B. ; Go, G. W. ; Johnson, B. J. ; Chung, K. Y. ; Choi, S. H. ; Sawyer, J. E. ; Silvey, D. T. ; Gilmore, L. A. ; Ghahramany, G. ; Kim, K. H. / Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of angus steers between 9 and 16 months of age. In: Journal of Animal Science. 2012 ; Vol. 90, No. 8. pp. 2505-2514.
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abstract = "We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous adipose tissue contains the greatest concentration of MUFA and lowest concentration of SFA. Therefore, we hypothesized that brisket subcutaneous adipose tissue depots would exhibit greater adipogenic gene expression over time than other major subcutaneous adipose tissue depots. Four Angus steers, each at 9, 12, 14, and 16 mo of age, were harvested and fresh subcutaneous adipose tissue samples were collected from over the brisket, chuck, rib, loin, sirloin, round, flank, and plate. Relative gene expression for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β), stearoylcoenzyme A desaturase (SCD), AMP-activated protein kinase alpha (AMPKα), and G-coupled protein receptor 43 (GPR43) was analyzed by quantitative real-time PCR. Expression of C/EBPβ, PPARγ, and CPT-1β was greatest at 12 to 14 mo of age (all P < 0.0001) and declined to very low abundance by 16 mo of age in all depots. Expression of PPARγ and CPT-1β was greater (P < 0.03) in flank, rib, and sirloin subcutaneous adipose tissues than in brisket and round adipose tissues. The expression of the SCD gene did not differ among the 4 age groups (P = 0.95). The palmitoleic: stearic acid ratio (an estimate of SCD activity) was greater (P < 0.001) in the subcutaneous adipose tissues from brisket, plate, and round than in the loin, rib, and sirloin. Conversely, subcutaneous adipose tissue from the loin, rib, and sirloin had greater (P < 0.001) SCD gene expression than the brisket, plate, and round. In general, subcutaneous adipose tissues with the highest concentration of MUFA and least SFA consistently exhibited the least SCD gene expression and adipogenic gene expression. We conclude that MUFA in the brisket and other depots with large SCD indices were deposited before 9 mo of age, during a time when the subcutaneous adipocytes were highly differentiated.",
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Smith, SB, Go, GW, Johnson, BJ, Chung, KY, Choi, SH, Sawyer, JE, Silvey, DT, Gilmore, LA, Ghahramany, G & Kim, KH 2012, 'Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of angus steers between 9 and 16 months of age', Journal of Animal Science, vol. 90, no. 8, pp. 2505-2514. https://doi.org/10.2527/jas.2011-4602

Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of angus steers between 9 and 16 months of age. / Smith, S. B.; Go, G. W.; Johnson, B. J.; Chung, K. Y.; Choi, S. H.; Sawyer, J. E.; Silvey, D. T.; Gilmore, L. A.; Ghahramany, G.; Kim, K. H.

In: Journal of Animal Science, Vol. 90, No. 8, 01.08.2012, p. 2505-2514.

Research output: Contribution to journalArticle

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T1 - Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of angus steers between 9 and 16 months of age

AU - Smith, S. B.

AU - Go, G. W.

AU - Johnson, B. J.

AU - Chung, K. Y.

AU - Choi, S. H.

AU - Sawyer, J. E.

AU - Silvey, D. T.

AU - Gilmore, L. A.

AU - Ghahramany, G.

AU - Kim, K. H.

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Y1 - 2012/8/1

N2 - We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous adipose tissue contains the greatest concentration of MUFA and lowest concentration of SFA. Therefore, we hypothesized that brisket subcutaneous adipose tissue depots would exhibit greater adipogenic gene expression over time than other major subcutaneous adipose tissue depots. Four Angus steers, each at 9, 12, 14, and 16 mo of age, were harvested and fresh subcutaneous adipose tissue samples were collected from over the brisket, chuck, rib, loin, sirloin, round, flank, and plate. Relative gene expression for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β), stearoylcoenzyme A desaturase (SCD), AMP-activated protein kinase alpha (AMPKα), and G-coupled protein receptor 43 (GPR43) was analyzed by quantitative real-time PCR. Expression of C/EBPβ, PPARγ, and CPT-1β was greatest at 12 to 14 mo of age (all P < 0.0001) and declined to very low abundance by 16 mo of age in all depots. Expression of PPARγ and CPT-1β was greater (P < 0.03) in flank, rib, and sirloin subcutaneous adipose tissues than in brisket and round adipose tissues. The expression of the SCD gene did not differ among the 4 age groups (P = 0.95). The palmitoleic: stearic acid ratio (an estimate of SCD activity) was greater (P < 0.001) in the subcutaneous adipose tissues from brisket, plate, and round than in the loin, rib, and sirloin. Conversely, subcutaneous adipose tissue from the loin, rib, and sirloin had greater (P < 0.001) SCD gene expression than the brisket, plate, and round. In general, subcutaneous adipose tissues with the highest concentration of MUFA and least SFA consistently exhibited the least SCD gene expression and adipogenic gene expression. We conclude that MUFA in the brisket and other depots with large SCD indices were deposited before 9 mo of age, during a time when the subcutaneous adipocytes were highly differentiated.

AB - We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous adipose tissue contains the greatest concentration of MUFA and lowest concentration of SFA. Therefore, we hypothesized that brisket subcutaneous adipose tissue depots would exhibit greater adipogenic gene expression over time than other major subcutaneous adipose tissue depots. Four Angus steers, each at 9, 12, 14, and 16 mo of age, were harvested and fresh subcutaneous adipose tissue samples were collected from over the brisket, chuck, rib, loin, sirloin, round, flank, and plate. Relative gene expression for C/EBPβ, PPARγ, carnitine palmitoyltransferase-1 beta (CPT-1β), stearoylcoenzyme A desaturase (SCD), AMP-activated protein kinase alpha (AMPKα), and G-coupled protein receptor 43 (GPR43) was analyzed by quantitative real-time PCR. Expression of C/EBPβ, PPARγ, and CPT-1β was greatest at 12 to 14 mo of age (all P < 0.0001) and declined to very low abundance by 16 mo of age in all depots. Expression of PPARγ and CPT-1β was greater (P < 0.03) in flank, rib, and sirloin subcutaneous adipose tissues than in brisket and round adipose tissues. The expression of the SCD gene did not differ among the 4 age groups (P = 0.95). The palmitoleic: stearic acid ratio (an estimate of SCD activity) was greater (P < 0.001) in the subcutaneous adipose tissues from brisket, plate, and round than in the loin, rib, and sirloin. Conversely, subcutaneous adipose tissue from the loin, rib, and sirloin had greater (P < 0.001) SCD gene expression than the brisket, plate, and round. In general, subcutaneous adipose tissues with the highest concentration of MUFA and least SFA consistently exhibited the least SCD gene expression and adipogenic gene expression. We conclude that MUFA in the brisket and other depots with large SCD indices were deposited before 9 mo of age, during a time when the subcutaneous adipocytes were highly differentiated.

KW - Adipose tissue

KW - Bovine

KW - Fatty acid

KW - Gene expression

KW - Growth

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