Acetoclastic methanogenesis led by Methanosarcina in anaerobic co-digestion of fats, oil and grease for enhanced production of methane

Mayur B. Kurade, Shouvik Saha, El Sayed Salama, Swapnil M. Patil, Sanjay P. Govindwar, Byong Hun Jeon

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Fats, oil and grease (FOG) are energy-dense wastes that substantially increase biomethane recovery. Shifts in the microbial community during anaerobic co-digestion of FOG was assessed to understand relationships between substrate digestion and microbial adaptations. Excessive addition of FOG inhibited the methanogenic activity during initial phase; however, it enhanced the ultimate methane production by 217% compared to the control. The dominance of Proteobacteria was decreased with a simultaneous increase in Firmicutes, Bacteriodetes, Synergistetes and Euryarchaeota during the co-digestion. A significant increase in Syntrophomonas (0.18–11%), Sporanaerobacter (0.14–6%) and Propionispira (0.02–19%) was observed during co-digestion, which substantiated their importance in acetogenesis. Among methanogenic Archaea, the dominance of Methanosaeta (94%) at the beginning of co-digestion was gradually replaced by Methanosarcina (0.52–95%). The absence/relatively low abundance of syntrophic acetate oxidizers and hydrogenotrophic methanogens, and dominance of acetoclastic methanogens suggested that methane generation during co-digestion of FOG was predominantly conducted through acetoclastic pathway led by Methanosarcina.

Original languageEnglish
Pages (from-to)351-359
Number of pages9
JournalBioresource Technology
Volume272
DOIs
StatePublished - 2019 Jan

Keywords

  • Anaerobic co-digestion
  • Fats, oil and grease (FOG)
  • High-throughput sequencing
  • Long-chain fatty acids
  • Methanosaeta
  • Methanosarcina

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