A highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes

K. Yugender Goud, V. Sunil Kumar, Akhtar Hayat, K. Vengatajalabathy Gobi, Hocheol Song, Ki-Hyun Kim, Jean Louis Marty

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL −1 ) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89–97% against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.

Original languageEnglish
Pages (from-to)336-342
Number of pages7
JournalJournal of Electroanalytical Chemistry
Volume832
DOIs
StatePublished - 2019 Jan 1

Fingerprint

Zearalenone
Immunosensors
Beer
Electrodes
Monoclonal antibodies
Wine
Phosphatases
Voltammetry
Fungi
Antibodies
Assays
Phosphates
Enzymes
Recovery
Carbon
Substrates
Mycotoxins
Bovine Serum Albumin
Alkaline Phosphatase
Monoclonal Antibodies

Keywords

  • BSA immobilization
  • Direct immobilization
  • Electrochemical immunosensor
  • Voltammetry
  • Zearalenone

Cite this

Yugender Goud, K. ; Sunil Kumar, V. ; Hayat, Akhtar ; Vengatajalabathy Gobi, K. ; Song, Hocheol ; Kim, Ki-Hyun ; Marty, Jean Louis. / A highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes. In: Journal of Electroanalytical Chemistry. 2019 ; Vol. 832. pp. 336-342.
@article{4cfc856065a1406e9cebb5bb726eb6ac,
title = "A highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes",
abstract = "Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL −1 ) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89–97{\%} against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.",
keywords = "BSA immobilization, Direct immobilization, Electrochemical immunosensor, Voltammetry, Zearalenone",
author = "{Yugender Goud}, K. and {Sunil Kumar}, V. and Akhtar Hayat and {Vengatajalabathy Gobi}, K. and Hocheol Song and Ki-Hyun Kim and Marty, {Jean Louis}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.jelechem.2018.10.058",
language = "English",
volume = "832",
pages = "336--342",
journal = "Journal of Electroanalytical Chemistry",
issn = "1572-6657",

}

A highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes. / Yugender Goud, K.; Sunil Kumar, V.; Hayat, Akhtar; Vengatajalabathy Gobi, K.; Song, Hocheol; Kim, Ki-Hyun; Marty, Jean Louis.

In: Journal of Electroanalytical Chemistry, Vol. 832, 01.01.2019, p. 336-342.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes

AU - Yugender Goud, K.

AU - Sunil Kumar, V.

AU - Hayat, Akhtar

AU - Vengatajalabathy Gobi, K.

AU - Song, Hocheol

AU - Kim, Ki-Hyun

AU - Marty, Jean Louis

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL −1 ) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89–97% against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.

AB - Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL −1 ) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89–97% against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.

KW - BSA immobilization

KW - Direct immobilization

KW - Electrochemical immunosensor

KW - Voltammetry

KW - Zearalenone

UR - http://www.scopus.com/inward/record.url?scp=85056836799&partnerID=8YFLogxK

U2 - 10.1016/j.jelechem.2018.10.058

DO - 10.1016/j.jelechem.2018.10.058

M3 - Article

VL - 832

SP - 336

EP - 342

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

SN - 1572-6657

ER -