γ-Fe2O3@SiO2 core-shell structured nanoparticle: Fabrication via surface treatment and application for plasmid DNA purification

Gye Seok An, Soo Wan Choi, Dong Ho Chae, Hyeon Seung Lee, Hyeong Jun Kim, Yoo Jin Kim, Yeon Gil Jung, Sung Churl Choi

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Abstract

Plasmid DNA was purified using core-shell γ-Fe2O3@SiO2 nanoparticles synthesized by a common sol-gel method utilizing tetraethylorthosilicate (TEOS) as a silica precursor. The dispersibility and TEOS binding affinity of γ-Fe2O3 nanoparticles were enhanced by acid/base treatment due to the formation of additional surface hydroxyl groups prior to SiO2 deposition. Since SiO2 was coated under basic conditions in the presence of ammonia as a catalyst, base-treated γ-Fe2O3 nanoparticles were more suited for this modification due to exhibiting a larger zeta potential and a lower saturation magnetization loss. The performance of the synthesized γ-Fe2O3@SiO2 nanoparticles with a 15-nm-thick SiO2 layer in the purification of plasmid DNA was compared to that of commercial Fe3O4-based magnetic beads. The used DNA samples exhibited similar purities, and all samples had similar theoretical plasmid DNA binding capacities. However, γ-Fe2O3@SiO2 nanoparticles exhibited a faster separation speed and a larger saturation magnetization of 100 emu/g compared to that of commercial Fe3O4-based magnetic beads (75 emu/g).

Original languageEnglish
Pages (from-to)12888-12892
Number of pages5
JournalCeramics International
Volume43
Issue number15
DOIs
Publication statusPublished - 2017 Oct 15

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Keywords

  • Core-shell structure
  • Maghemite
  • Plasmid DNA purification
  • Surface treatment
  • Zeta potential

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